| Antibodies |
|
| Phospho-Akt (Ser473), clone D9E |
Cell Signaling Technology |
Cat # 4060 RRID: AB_2315049
|
| Akt (pan), clone 40D4 |
Cell Signaling Technology |
Cat #2920 RRID: AB_1147620
|
| Phospho-anti FoxO3a (Ser253)/FoxO1 (Ser251), clone D18H8 |
Cell Signaling Technology |
Cat #13129 RRID: AB_2687495
|
| β-Actin (13E5) Rabbit |
Cell Signaling Technology |
Cat #4970 RRID AB_2223172
|
| Anti-FoxO1, clone C29H4 |
Cell Signaling Technology |
Cat #2880 RRID: AB_2106495
|
| p27/ Kip1 (D69C12) |
Cell Signaling Technology |
Cat# 3686 RRID:AB_2077850
|
| Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 |
Thermofisher Scientific |
Cat# A-11008, RRID:AB_143165
|
| PerCP anti-mouse CD3epsilon antibody |
BioLegend |
Cat# 100326, RRID:AB_893317
|
| CD4 antibody |
BD Biosciences |
Cat# 563790, RRID:AB_2738426
|
| BUV805 Rat Anti-Mouse CD8a antibody |
BD Biosciences |
Cat# 564920, RRID:AB_2716856
|
| CD11b Monoclonal Antibody (M1/70), PE |
Thermo Fisher Scientific |
Cat# 12-0112-82, RRID:AB_2734869
|
| CD11c Monoclonal Antibody (N418), FITC |
Thermo Fisher Scientific |
Cat# 11-0114-82, RRID:AB_464940
|
| CD25 Monoclonal Antibody (PC61.5), PE |
Thermo Fisher Scientific |
Cat# 12-0251-81, RRID:AB_465606
|
| CD44 Monoclonal Antibody (IM7), PE-Cyanine7 |
Thermo Fisher Scientific |
Cat# 25-0441-81, RRID:AB_469622
|
| BV786 Rat Anti-Mouse CD45 antibody |
BD Biosciences |
Cat# 564225, RRID:AB_2716861
|
| CD62L (L-Selectin) Monoclonal Antibody (MEL-14), APC |
Thermo Fisher Scientific |
Cat# 17-0621-82, RRID:AB_469410
|
| CD206 (MMR) Monoclonal Antibody (MR6F3),APC |
Thermo Fisher Scientific |
Cat# 17-2061-82, RRID:AB_2637420
|
| F4/80 Monoclonal Antibody (BM8), PE-Cyanine7 |
Thermo Fisher Scientific |
Cat# 25-4801-82, RRID:AB_469653
|
| Armenian Hamster Anti-CD69 Monoclonal Antibody, FITC Conjugated, Clone H1.2F3 |
BD Biosciences |
Cat# 553236, RRID:AB_394725
|
| FOXP3 Monoclonal Antibody (FJK-16s), FITC |
Thermo Fisher Scientific |
Cat# 11-5773-80, RRID:AB_465242
|
| FITC anti-human/mouse Granzyme B antibody |
BioLegend |
Cat# 515403, RRID:AB_2114575
|
| Ly-6C Monoclonal Antibody (HK1.4), APC-eFluor 780 |
Thermo Fisher Scientific |
Cat# 47-5932-82, RRID:AB_2573992
|
| BUV395 Rat Anti-Mouse Ly-6G antibody |
BD Biosciences |
Cat# 563978, RRID:AB_2716852
|
| Ms I-A/I-E BUV805 M5/114.15.2 antibody |
BD Biosciences |
Cat# 748844, RRID:AB_2873247
|
|
| Chemicals, peptides, and recombinant proteins |
|
| Alexa Fluor™ 568 Phalloidin |
Thermofisher Scientific |
Cat#A12380 |
| Viability Dye |
ThermoFisher Scientific |
Cat#65-0863 |
| DMEM |
Gibco |
Cat# 11965084 |
| RPMI 1640 Medium |
Gibco |
Cat# 11875119 |
| IACS-010759 |
Cayman Chemical |
Cat# 25867 |
| TMRE |
Thermofisher Scientific |
Cat# T669 |
| InVivoPlus anti-mouse CD8α |
Bio X Cell |
Cat# BP0061 |
| InVivoPlus anti-mouse CD4α |
Bio X Cell |
Cat# BP0003 |
| InVivoPlus rat IgG2 k |
Bio X Cell |
Cat# BP0090 |
| Red Blood Lysis Buffer |
Biolegend |
Cat# 420301 |
| Recombinant murine M-CSF |
Peprotech |
Cat# 315-02 |
| Recombinant human IL-2 |
Peprotech |
Cat# 200-02 |
| Recombinant murine IL-6 |
Peprotech |
Cat# 216-16 |
| Recombinant murine GM-CSF |
Peprotech |
Cat# 315-03 |
| Recombinant murine G-CSF |
Peprotech |
Cat# 250-05 |
| Triphenylphosphine |
Sigma-Aldrich |
Cat#603-35-0 |
| 1,10-di-bromo-decane |
Alfa Aesar |
Cat#AAA1235722 |
| CH2Cl2-EtOH |
Fisher Scientific Co |
Cat#AC124050010 |
| CH2Cl2-EtOH |
Sigma-Aldrich |
Cat#75-09-2 |
| Magnolol |
ASTATECH |
Cat#528-43-8 |
| EverBrite™ Mounting Medium |
Biotium |
Cat#23001 |
| DMF |
Fisher Scientific Co |
Cat#D119-500 |
|
| Critical commercial assays |
|
| MOJO sort Kit |
Biolegend |
Cat# 480024 |
| Dynabeads™ Mouse T-Activator CD3/CD28 |
Thermofisher Scientific |
Cat# 1456D |
| Cell Trace Violet |
Thermofisher Scientific |
Cat# C34557
|
| Foxp3 Transcription Factor Staining Buffer |
eBioscience |
Cat# 00-5523-00 |
| DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) |
Thermofisher Scientific |
Cat#D1306 |
| Propidium iodide / RNase solution |
Thermofisher Scientific |
Cat#F10797
|
| Image J |
|
RRID: SCR_003070 |
| CellProfile PipeLine for immunofluorescence analyses |
This Paper |
https://github.com/Hill-Lab/Mito-MGN-Scripts |
|
| Experimental models: cell lines |
|
| Male murine melanoma B16.F0 |
ATCC |
Cat#CRL-6322; RRID:CVCL_0604 |
| Male murine melanoma B16.F0 |
ATCC |
Cat# CRL-6475; RRID:CVCL_0159 |
|
| Software and algorithms |
|
| ImageJ |
Schindelin et al., 2012 |
https://imagej.nih.gov/ij/ |
| CellProfiler |
McQuin et al., 2018 |
https://cellprofiler.org/ |
|
| Other |
|
| C57BL/6 |
The Jackson Laboratories |
Cat#0000664; RRID:IMSR_JAX:000664 |