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. 2021 May 25;24(6):102653. doi: 10.1016/j.isci.2021.102653
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Phospho-Akt (Ser473), clone D9E Cell Signaling Technology Cat # 4060 RRID: AB_2315049
Akt (pan), clone 40D4 Cell Signaling Technology Cat #2920 RRID: AB_1147620
Phospho-anti FoxO3a (Ser253)/FoxO1 (Ser251), clone D18H8 Cell Signaling Technology Cat #13129 RRID: AB_2687495
β-Actin (13E5) Rabbit Cell Signaling Technology Cat #4970 RRID AB_2223172
Anti-FoxO1, clone C29H4 Cell Signaling Technology Cat #2880 RRID: AB_2106495
p27/ Kip1 (D69C12) Cell Signaling Technology Cat# 3686 RRID:AB_2077850
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermofisher Scientific Cat# A-11008, RRID:AB_143165
PerCP anti-mouse CD3epsilon antibody BioLegend Cat# 100326, RRID:AB_893317
CD4 antibody BD Biosciences Cat# 563790, RRID:AB_2738426
BUV805 Rat Anti-Mouse CD8a antibody BD Biosciences Cat# 564920, RRID:AB_2716856
CD11b Monoclonal Antibody (M1/70), PE Thermo Fisher Scientific Cat# 12-0112-82, RRID:AB_2734869
CD11c Monoclonal Antibody (N418), FITC Thermo Fisher Scientific Cat# 11-0114-82, RRID:AB_464940
CD25 Monoclonal Antibody (PC61.5), PE Thermo Fisher Scientific Cat# 12-0251-81, RRID:AB_465606
CD44 Monoclonal Antibody (IM7), PE-Cyanine7 Thermo Fisher Scientific Cat# 25-0441-81, RRID:AB_469622
BV786 Rat Anti-Mouse CD45 antibody BD Biosciences Cat# 564225, RRID:AB_2716861
CD62L (L-Selectin) Monoclonal Antibody (MEL-14), APC Thermo Fisher Scientific Cat# 17-0621-82, RRID:AB_469410
CD206 (MMR) Monoclonal Antibody (MR6F3),APC Thermo Fisher Scientific Cat# 17-2061-82, RRID:AB_2637420
F4/80 Monoclonal Antibody (BM8), PE-Cyanine7 Thermo Fisher Scientific Cat# 25-4801-82, RRID:AB_469653
Armenian Hamster Anti-CD69 Monoclonal Antibody, FITC Conjugated, Clone H1.2F3 BD Biosciences Cat# 553236, RRID:AB_394725
FOXP3 Monoclonal Antibody (FJK-16s), FITC Thermo Fisher Scientific Cat# 11-5773-80, RRID:AB_465242
FITC anti-human/mouse Granzyme B antibody BioLegend Cat# 515403, RRID:AB_2114575
Ly-6C Monoclonal Antibody (HK1.4), APC-eFluor 780 Thermo Fisher Scientific Cat# 47-5932-82, RRID:AB_2573992
BUV395 Rat Anti-Mouse Ly-6G antibody BD Biosciences Cat# 563978, RRID:AB_2716852
Ms I-A/I-E BUV805 M5/114.15.2 antibody BD Biosciences Cat# 748844, RRID:AB_2873247

Chemicals, peptides, and recombinant proteins

Alexa Fluor™ 568 Phalloidin Thermofisher Scientific Cat#A12380
Viability Dye ThermoFisher Scientific Cat#65-0863
DMEM Gibco Cat# 11965084
RPMI 1640 Medium Gibco Cat# 11875119
IACS-010759 Cayman Chemical Cat# 25867
TMRE Thermofisher Scientific Cat# T669
InVivoPlus anti-mouse CD8α Bio X Cell Cat# BP0061
InVivoPlus anti-mouse CD4α Bio X Cell Cat# BP0003
InVivoPlus rat IgG2 k Bio X Cell Cat# BP0090
Red Blood Lysis Buffer Biolegend Cat# 420301
Recombinant murine M-CSF Peprotech Cat# 315-02
Recombinant human IL-2 Peprotech Cat# 200-02
Recombinant murine IL-6 Peprotech Cat# 216-16
Recombinant murine GM-CSF Peprotech Cat# 315-03
Recombinant murine G-CSF Peprotech Cat# 250-05
Triphenylphosphine Sigma-Aldrich Cat#603-35-0
1,10-di-bromo-decane Alfa Aesar Cat#AAA1235722
CH2Cl2-EtOH Fisher Scientific Co Cat#AC124050010
CH2Cl2-EtOH Sigma-Aldrich Cat#75-09-2
Magnolol ASTATECH Cat#528-43-8
EverBrite™ Mounting Medium Biotium Cat#23001
DMF Fisher Scientific Co Cat#D119-500

Critical commercial assays

MOJO sort Kit Biolegend Cat# 480024
Dynabeads™ Mouse T-Activator CD3/CD28 Thermofisher Scientific Cat# 1456D
Cell Trace Violet Thermofisher Scientific Cat# C34557
Foxp3 Transcription Factor Staining Buffer eBioscience Cat# 00-5523-00
DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Thermofisher Scientific Cat#D1306
Propidium iodide / RNase solution Thermofisher Scientific Cat#F10797
Image J RRID: SCR_003070
CellProfile PipeLine for immunofluorescence analyses This Paper https://github.com/Hill-Lab/Mito-MGN-Scripts

Experimental models: cell lines

Male murine melanoma B16.F0 ATCC Cat#CRL-6322; RRID:CVCL_0604
Male murine melanoma B16.F0 ATCC Cat# CRL-6475; RRID:CVCL_0159

Software and algorithms

ImageJ Schindelin et al., 2012 https://imagej.nih.gov/ij/
CellProfiler McQuin et al., 2018 https://cellprofiler.org/

Other

C57BL/6 The Jackson Laboratories Cat#0000664; RRID:IMSR_JAX:000664