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. 2021 Jun 16;2(3):100609. doi: 10.1016/j.xpro.2021.100609

Table 2.

Spike in normalization for H3K4me2 in murine macrophages after LPS (L) or LPS+ Dexamethasone (L+D) treatment

Raw Ct value
%input
Fkbp5 NegPol2 eRF3 Fkbp5 NegPol2 eRF3
IgG1 L+D_1 32.32 30.17 32.00 0.047 0.080 0.042
IgG1 L+D_2 33.55 29.66 32.27 0.020 0.115 0.035
IgG1 L+D_3 32.37 29.51 31.20 0.045 0.127 0.074
IgG2 L+D_1 34.88 29.76 32.23 0.008 0.107 0.036
IgG2 L+D_2 33.26 30.92 32.01 0.024 0.048 0.042
IgG2 L+D_3 33.31 29.70 32.4 0.023 0.112 0.032
H3K4me2_1 L+D_1 23.10 27.66 23.41 28.087 0.459 16.429
H3K4me2_2 L+D_2 22.78 27.25 23.51 35.062 0.610 15.273
H3K4me2_3 L+D_3 23.17 27.24 23.54 26.850 0.617 14.950
H3K4me2_1 L+D_1 25.25 28.79 25.31 6.324 0.210 4.407
H3K4me2_2 L+D_2 24.57 28.39 25.11 10.146 0.277 5.028
H3K4me2_3 L+D_3 24.48 29.28 25.05 10.829 0.150 5.256
Input L+D 50%_1 22.35 20.93 21.78
Input L+D 50%_2 22.33 20.85 21.78
Input L+D 50%_3 22.13 20.92 21.85
IgG1 L_1 33.37 29.59 37.74 0.015 0.082 0.000
IgG1 L_2 33.33 29.24 43.52 0.015 0.105 0.000
IgG1 L_3 32.53 29.46 31.86 0.027 0.090 0.042
IgG2 L_1 34.91 32.30 32.41 0.005 0.012 0.029
IgG2 L_2 32.79 30.84 33.37 0.022 0.034 0.014
IgG2 L_3 33.62 30.47 32.93 0.012 0.045 0.020
H3K4me2_1 L_1 24.20 27.85 25.01 8.847 0.277 4.907
H3K4me2_2 L_2 24.23 28.13 25.07 8.628 0.229 4.702
H3K4me2_3 L_3 24.17 27.51 24.83 9.026 0.352 5.553
H3K4me2_1 L_1 22.92 26.54 23.62 21.409 0.686 12.888
H3K4me2_2 L_2 22.88 27.49 23.71 22.057 0.356 12.138
H3K4me2_3 L_3 22.84 27.20 23.69 22.646 0.436 12.263
Input L_1 50% 21.64 20.22 21.34
Input L_2 50% 21.66 20.30 21.43
Input L_3 50% 21.79 20.56 22.22

Mean of Input Samples

Fkbp5 NegPol2 eRF3
Input L+D 22.270 20.897 21.801
Input L 21.698 20.359 21.664

Adjusted Input to 100%

Fkbp5 NegPol2 eRF3
Input L+D 21.270 19.897 20.801
Input L 20.698 19.359 20.664

Raw Ct values and %input.