Table 2.
Spike in normalization for H3K4me2 in murine macrophages after LPS (L) or LPS+ Dexamethasone (L+D) treatment
| Raw Ct value |
%input |
|||||
|---|---|---|---|---|---|---|
| Fkbp5 | NegPol2 | eRF3 | Fkbp5 | NegPol2 | eRF3 | |
| IgG1 L+D_1 | 32.32 | 30.17 | 32.00 | 0.047 | 0.080 | 0.042 |
| IgG1 L+D_2 | 33.55 | 29.66 | 32.27 | 0.020 | 0.115 | 0.035 |
| IgG1 L+D_3 | 32.37 | 29.51 | 31.20 | 0.045 | 0.127 | 0.074 |
| IgG2 L+D_1 | 34.88 | 29.76 | 32.23 | 0.008 | 0.107 | 0.036 |
| IgG2 L+D_2 | 33.26 | 30.92 | 32.01 | 0.024 | 0.048 | 0.042 |
| IgG2 L+D_3 | 33.31 | 29.70 | 32.4 | 0.023 | 0.112 | 0.032 |
| H3K4me2_1 L+D_1 | 23.10 | 27.66 | 23.41 | 28.087 | 0.459 | 16.429 |
| H3K4me2_2 L+D_2 | 22.78 | 27.25 | 23.51 | 35.062 | 0.610 | 15.273 |
| H3K4me2_3 L+D_3 | 23.17 | 27.24 | 23.54 | 26.850 | 0.617 | 14.950 |
| H3K4me2_1 L+D_1 | 25.25 | 28.79 | 25.31 | 6.324 | 0.210 | 4.407 |
| H3K4me2_2 L+D_2 | 24.57 | 28.39 | 25.11 | 10.146 | 0.277 | 5.028 |
| H3K4me2_3 L+D_3 | 24.48 | 29.28 | 25.05 | 10.829 | 0.150 | 5.256 |
| Input L+D 50%_1 | 22.35 | 20.93 | 21.78 | |||
| Input L+D 50%_2 | 22.33 | 20.85 | 21.78 | |||
| Input L+D 50%_3 | 22.13 | 20.92 | 21.85 | |||
| IgG1 L_1 | 33.37 | 29.59 | 37.74 | 0.015 | 0.082 | 0.000 |
| IgG1 L_2 | 33.33 | 29.24 | 43.52 | 0.015 | 0.105 | 0.000 |
| IgG1 L_3 | 32.53 | 29.46 | 31.86 | 0.027 | 0.090 | 0.042 |
| IgG2 L_1 | 34.91 | 32.30 | 32.41 | 0.005 | 0.012 | 0.029 |
| IgG2 L_2 | 32.79 | 30.84 | 33.37 | 0.022 | 0.034 | 0.014 |
| IgG2 L_3 | 33.62 | 30.47 | 32.93 | 0.012 | 0.045 | 0.020 |
| H3K4me2_1 L_1 | 24.20 | 27.85 | 25.01 | 8.847 | 0.277 | 4.907 |
| H3K4me2_2 L_2 | 24.23 | 28.13 | 25.07 | 8.628 | 0.229 | 4.702 |
| H3K4me2_3 L_3 | 24.17 | 27.51 | 24.83 | 9.026 | 0.352 | 5.553 |
| H3K4me2_1 L_1 | 22.92 | 26.54 | 23.62 | 21.409 | 0.686 | 12.888 |
| H3K4me2_2 L_2 | 22.88 | 27.49 | 23.71 | 22.057 | 0.356 | 12.138 |
| H3K4me2_3 L_3 | 22.84 | 27.20 | 23.69 | 22.646 | 0.436 | 12.263 |
| Input L_1 50% | 21.64 | 20.22 | 21.34 | |||
| Input L_2 50% | 21.66 | 20.30 | 21.43 | |||
| Input L_3 50% | 21.79 | 20.56 | 22.22 | |||
| Mean of Input Samples | ||||||
| Fkbp5 | NegPol2 | eRF3 | ||||
| Input L+D | 22.270 | 20.897 | 21.801 | |||
| Input L | 21.698 | 20.359 | 21.664 | |||
| Adjusted Input to 100% | ||||||
| Fkbp5 | NegPol2 | eRF3 | ||||
| Input L+D | 21.270 | 19.897 | 20.801 | |||
| Input L | 20.698 | 19.359 | 20.664 | |||
Raw Ct values and %input.