5% BSA	Final concentration	Amount
BSA	5% w/v	2.5 g
Nuclease-free water	N/A	50 mL
Total		50 mL

Filter the buffer with a 0.45 μm syringe filter. Aliquot and store 5% BSA at −20°C for up to one year. Aliquots might be thawed for up to 5 times.