Figure 1.
Changes in skeletal growth patterns in coral primary polyps with decreasing pH. (a–c) Microscopy images showing the Calcein Blue fluorescence (expressed as percentage intensity) in the skeleton of the primary polyps at (a) pH 8.2, (b) pH 7.8 and (c) pH 7.6. Magnification: 4×. Scale bar: 400 µm. (d–f) Micro-CT images showing top views of three-dimensional reconstructions of the primary polyps' skeleton at (d) pH 8.2, (e) pH 7.8 and (f) pH 7.6. Scale bar: 400 µm. (g–i) Slices of the three-dimensional reconstructions showing thresholded two-dimensional slices of the base of the septa at (g) pH 8.2, (h) pH 7.8 and (i) pH 7.6. Scale bar: 200 µm. (j–l) Skeletal thickness distribution (μm) along the vertical axis of the septa of the primary polyps at (j) pH 8.2, (k) pH 7.8 and (l) pH 7.6. Scale bar: 200 µm. (Online version in colour.)
