REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-V5 tag, clone SV5-Pk1	Bio-Rad	MCA1360
Mouse anti-FLAG, clone M2	Sigma	F-3165
Rabbit anti-NEMF	Thermo	PA5-36308
Rabbit anti-Rpl23	Abcam	ab112587
Rabbit anti-Rpl26	Bethyl Laboratories	A300-686A
Rabbit anti-Rps3	Bethyl Laboratories	A303-841A
Mouse anti-GAPDH	MBL	M171-3
Chemicals, peptides, and recombinant proteins
DMEM medium	Nacalai	08458-16
FBS	Thermo	26140-079
0.25% Trypsin-EDTA	Nacalai	32777-44
Opti-MEM reduced serum medium	Thermo	31985070
PEI-Max reagent	Polysciences	24765-1
Cycloheximide	Nacalai	06741-04
cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail	Roche	11836170001
RNasin RNase inhibitor	Promega	N2115
D-PBS powder	Nacalai	07269-84
Protein G Dynabeads	Thermo	10003D
AcTEV Protease	Thermo	12575015
HEPES	Wako	340-01376
NaCl	Nacalai	31320-76
MgCl2	Wako	136-03995
Igepal CA-630	Sigma	I8896
Sucrose	Nacalai	30406-25
DTT	Nacalai	14128-91
β-Mercaptoethanol	Nacalai	21438-82
Glycerol	Wako	072-04945
Acidic phenol	Wako	315-90291
Chloroform	Wako	038-02606
20 mg/mL Glycogen Solution	Wako	076-06621
3 M Sodium acetate	Ambion	AM9740
Ethanol	Wako	057-00451
Critical commercial assays
ReverTra Ace RT Kit	Toyobo	TRT-101
KAPA SYBR FAST qPCR Mix	NIPPON Genetics	KK4602
Experimental models: cell lines
HEK293T	ATCC	CRL-11268
HeLa	ATCC	CCL-2
Oligonucleotides
Ala-TGC-F: 5′-TGGTAGAGCGCATGCTTTGC-3′	FASMAC	Custom order
Ala-TGC-R: 5′-AACCCGGGGCCTCATACATG-3′	FASMAC	Custom order
Ala-AGC-F: 5′-AGTGGTAGAGCGCGTGCTTA-3′	FASMAC	Custom order
Ala-AGC-R: 5′-CCGGGGCCTCGTGCATGCT-3′	FASMAC	Custom order
Ala-CGC-F: 5′-TGGTAGAGCGCATGCTTCGC-3′	FASMAC	Custom order
Ala-CGC-R: 5′-AACCCGGGACCTCATACATG-3′	FASMAC	Custom order
Thr-AGT-F: 5′-GGTTAAAGCGCCTGTCTAGT-3′	FASMAC	Custom order
Thr-AGT-R: 5′-AACCCAGGATCTCCTGTTTA-3′	FASMAC	Custom order
Thr-CGT-F: 5′-TGGTAAGGCGTCGGTCTCGT-3′	FASMAC	Custom order
Thr-CGT-R: 5′-AACCCGCGATCTTCGGTTTA-3′	FASMAC	Custom order
Gly-TCC-F: 5′-GGTGAGCATAGCTGCCTTCC-3′	FASMAC	Custom order
Gly-TCC-R: 5′-GAACCCGGGTCAACTGCTTG-3′	FASMAC	Custom order
Gly-CCC-F: 5′-TGGTAGAATTCTCGCCTCCC-3′	FASMAC	Custom order
Gly-CCC-R: 5′-GAACCCGGGTCTCCCGCGTG-3′	FASMAC	Custom order
Gly-GCC-F: 5′-TGGTAGAATTCTCGCCTGCC-3′	FASMAC	Custom order
Gly-GCC-R: 5′-GAACCCGGGCCTCCCGCGTG-3′	FASMAC	Custom order
Lys-TTT-F: 5′-GTCGGTAGAGCATCAGACTT-3′	FASMAC	Custom order
Lys-TTT-R: 5′-CCTGGAACCCTCAGATTAAA-3′	FASMAC	Custom order
Lys-CTT-F: 5′-CGGTAGAGCATGAGACTCTT-3′	FASMAC	Custom order
Lys-CTT-R: 5′-AACCCATGACCCTGAGATTA-3′	FASMAC	Custom order
Asn-GTT-F: 5′-GGTTAGCGCGTTCGGCTGTT-3′	FASMAC	Custom order
Asn-GTT-R: 5′-AACCACCAACCTTTCGGTTA-3′	FASMAC	Custom order
Asp-GTC-F: 5′-GGTGAGTATCCCCGCCTGTC-3′	FASMAC	Custom order
Asp-GTC-R: 5′-GAACCCCGGTCTCCCGCGTG-3′	FASMAC	Custom order
Gln-CTG-F: 5′-GGTTAGCACTCTGGACTCTG-3′	FASMAC	Custom order
Gln-CTG-R: 5′-GAACTCGGATCGCTGGATTC-3′	FASMAC	Custom order
Glu-CTC-F: 5′-GGTTAGGATTCGGCGCTCTC-3′	FASMAC	Custom order
Glu-CTC-R: 5′-GAACCCGGGCCGCGGCGGTG-3′	FASMAC	Custom order
Pro-AGG-F: 5′-GGGTATGATTCTCGCTTAGG-3′	FASMAC	Custom order
Pro-AGG-R: 5′-AACCCGGGACCTCTCGCACC-3′	FASMAC	Custom order
Phe-GAA-F: 5′-TGGGAGAGCGTTAGACTGAA-3′	FASMAC	Custom order
Phe-GAA-R: 5′-AACCAGGGACCTTTAGATCT-3′	FASMAC	Custom order
Val-AAC-F: 5′-GGTTATCACGTTCGCCTAAC-3′	FASMAC	Custom order
Val-AAC-R: 5′-AACCGGGGACCTTTCGCGTG-3′	FASMAC	Custom order
Tyr-GTA-F: 5′-TGGTAGAGCGGAGGACTGTA-3′	FASMAC	Custom order
Tyr-GTA-R: 5′-AACCAGCGACCTAAGGATCT-3′	FASMAC	Custom order
Trp-CCA-F: 5′-CGGTAGCGCGTCTGACTCCA-3′	FASMAC	Custom order
Trp-CCA-R: 5′-AACACGCAACCTTCTGATCT-3′	FASMAC	Custom order
Leu-CAG-F: 5′-GTCTAAGGCGCTGCGTTCAG-3′	FASMAC	Custom order
Leu-CAG-R: 5′-CTCCAGGGGAGACTGCGACC-3′	FASMAC	Custom order
Ile-AAT-F: 5′-GGTTAGAGCGTGGTGCTAAT-3′	FASMAC	Custom order
Ile-AAT-R: 5′-AACCCGCGACCTTGGCGTTA-3′	FASMAC	Custom order
Cys-GCA-F: 5′-GGTAGAGCATTTGACTGCAG-3′	FASMAC	Custom order
Cys-GCA-R: 5′-GAACCAGGGACCTCTTGATC-3′	FASMAC	Custom order
Met-CAT-F: 5′-AGGCAGCGCGTCAGTCTCAT-3′	FASMAC	Custom order
Met-CAT-R: 5′-AACTCACGACCTTCAGATTA-3′	FASMAC	Custom order
His-GTG-F: 5′-GGTTAGTACTCTGCGTTGTG-3′	FASMAC	Custom order
His-GTG-R: 5′-GAACCGAGGTTGCTGCGGCC-3′	FASMAC	Custom order
Ser-AGA-F: 5′-GGTTAAGGCGATGGACTAGA-3′	FASMAC	Custom order
Ser-AGA-R: 5′-GGGGAGACCCCAATGGATTT-3′	FASMAC	Custom order
Ser-CGA-F: 5′-GGTTAAGGCGTTGGACTCGA-3′	FASMAC	Custom order
Ser-CGA-R: 5′-GGGGAGACCCCATTGGATTT-3′	FASMAC	Custom order
Arg-TCT-F: 5′-ATGGATAGCGCATTGGACTT-3′	FASMAC	Custom order
Arg-TCT-R: 5′-CCCACAACCTTTGAATTAGA-3′	FASMAC	Custom order
Arg-TCG-F: 5′-ATGGATAAGGCGTCTGACTT-3′	FASMAC	Custom order
Arg-TCG-R: 5′-CCCTCAATCTTCTGATCCGA-3′	FASMAC	Custom order
Arg-CCG-F: 5′-AATGGATAAGGCGTCTGATT-3′	FASMAC	Custom order
Arg-CCG-R: 5′-CCCTCAATCTTCTGATCCGG-3′	FASMAC	Custom order
28S rRNA-F: 5′-CAGGGGAATCCGACTGTTTA-3′	FASMAC	Custom order
28S rRNA-R: 5′-ATGACGAGGCATTTGGCTAC-3′	FASMAC	Custom order
Recombinant DNA
pcDNA-V5-TEV-NEMF	Udagawa et al., 2021	N/A
pcDNA-V5-NEMF	Udagawa et al., 2021	N/A
pcDNA-V5-TEV-NEMF D96A	Udagawa et al., 2021	N/A
p5FBG-Nonstop	Saito et al., 2013	N/A
Other
245 mm (500 cm2) Square dish	Corning	431110
Plastic dough scraper	Amazon (nescope)	B08KQG77CP
Open-top polyclear tubes for SW28 rotor	Seton	7052
Gradient Master	BioComp	108
Tube holder, long cap, and marker block for SW28	BioComp	105-925-R
Piston Gradient Fractionator	BioComp	152
Ultracentrifuge	Beckman	XL-A
Magnetic rack	Bio-Rad	1614916
96-Well plate	Sorenson	23080
Nanodrop	Thermo	ND-ONE-W
CFX Connect Real-Time PCR System	Bio-Rad	1855201J1