REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
4-(2-Hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES)	Lonza	Cat#BE17-737E; CAS: 7365-45-9
Bovine Serum Albumin	Sigma-Aldrich	Cat#B6917; CAS: 9048-46-8
Diethyl dicarbonate (DEPC)	Sigma-Aldrich	Cat#D5758; CAS: 1609-47-8
DL-Dithiothreitol (DTT)	New England Biolabs	Cat#B1034A; CAS: 3483-12-3
IGEPAL CA-630	Sigma-Aldrich	Cat#I8896; CAS: 9002-93-1
Magnesium chloride (MgCl2)	Sigma-Aldrich	Cat#M1028; CAS: 7786-30-3
Phosphate buffered saline (PBS) (10×, pH 7.2)	Gibco	Cat#70013-016
Potassium chloride (KCl)	Sigma-Aldrich	Cat#P9541; CAS: 7447-40-7
RNase Inhibitor, Murine	New England Biolabs	Cat#M0314
RNaseZAP	Sigma-Aldrich	Cat#R2020
Sucrose	Sigma-Aldrich	Cat#S0389; CAS: 57-50-1
Trypan Blue	Bio-Rad	Cat#1450013
Biological samples
Mouse white adipose tissues	N/A	N/A
Critical commercial assays
Chromium Next GEM Chip G Single Cell Kit (48 rxns)	10x Genomics	Cat#1000120
Chromium Next GEM Single Cell 3’ Kit v3.1 (16 rxns)	10x Genomics	Cat#1000268
Dual Index Kit TT Set A (for Gene Expression Libraries)	10x Genomics	Cat#1000215
Experimental models: organisms/strains
Mus musculus C57BL/6J and C57BL/6N	N/A	N/A
Other
0.2 μm Syringe filters	Sartorius	Cat#17845-ACK
1.5 mL DNA LoBind tubes	Eppendorf	Cat#0030108051
2 mL Dounce homogenizer	Sigma-Aldrich	Cat#D8938
5 mL DNA LoBind tubes	Eppendorf	Cat#0030122348
6 cm Petri dish	Thermo Scientific	Cat#150288
50 mL Tube	SARSTEDT	Cat#62.547.254
10× Genomics Chromium Controller	10x Genomics	Cat#PN110203; RRID:SCR_019326
BRAND counting chamber BLAUBRAND Bürker pattern	Sigma-Aldrich	Cat#BR718920
Flowmi Cell Strainer, porosity 40 μm	Sigma-Aldrich	Cat#BAH136800040
NovaSeq 6000 System	Illumina	Cat#20012850; RRID:SCR_016387
pluriStrainer 70 μm (Cell Strainer)	pluriSelect	Cat#43-50070-51
Surgical Scalpel Blade No.22	Swann-Morton	Cat#0208