HPG incorporation in hepatocytes with various HPG concentrations and incubation times
(A) Hepatocytes were incubated with Group I, 0 μM HPG for 10 min, 30 min and 60 min; Group II, 25 μM HPG for 10 min, 30 min and 60 min; Group III, 50 μM HPG for 10 min, 30 min and 60 min; Group IV, 100 μM HPG for 10 min, 30 min and 60 min. Scale bar, 50 μm.
(B) Quantification for A. Relative HPG incorporation is presented as the HPG: DAPI ratio ± SD, which were normalized to HPG(100 μM, 60 min) treatment (100%) (n ≥ 25).