REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Cycloheximide (CHX)	MCE	Cat#HY-12320
Collagenase	Sigma	Cat#C-6885
DMEM medium	Wisent Inc	Cat#319-005-CL
Fetal Bovine Serum (FBS)	Royacel	Cat#RY-F22-05
100 X penicillin-streptomycin	Wisent Inc	Cat#450-201-EL
PBS	Wisent Inc	Cat#311-010-CL
L-Glutamine	Sangon Biotech	Cat#E607004-0100
L-Cystine	Sangon Biotech	Cat#A610088-0100
DMEM L-methionine-free medium	Gibco	Cat#21013024
BSA	Sangon Biotech	Cat#A602440-0050
Triton X-100	Sangon Biotech	Cat#A110694-0500
NaCl	Sangon Biotech	Cat#A100241-0500
NaHCO3	Sangon Biotech	Cat#A610482-0500
Glucose	Sangon Biotech	Cat#A501991-0500
1 M HEPES	Sangon Biotech	Cat#E607018-0100
KCl	Sangon Biotech	Cat#A100395-0100
MgSO4·7H2O	Sangon Biotech	Cat#A500864-0500
KH2PO4	Sangon Biotech	Cat#A100781-0100
EGTA	Sangon Biotech	Cat#A600077-0025
CaCl2	Sangon Biotech	Cat#A100556-0250
Antifade Mounting Medium with DAPI	Vector Laboratories	Cat#H-1500
DAPI staining solution	Sangon Biotech	Cat#E607303
Trypan blue solution	Sigma	Cat#T8154
Paraformaldehyde (PFA)	Aladdin	Cat#C104188
Chloral hydrate	Sangon Biotech	Cat# A600288
Critical commercial assays
Click-iT® HPG Alexa Fluor® Protein Synthesis Assay Kits	Invitrogen	Cat#C10429
Experimental models: cell lines
Primary hepatocytes	This paper	N/A
Experimental models: organisms/strains
C57BL/6J	Jackson Laboratory	Cat#JAX:000664, RRID:IMSR_JAX:000664
Software and algorithms
Adobe Illustrator	Adobe	https://www.adobe.com/products/illustrator.html
Zeiss LSM Image Browser	Zeiss	https://www.zeiss.com/microscopy/int/downloads/lsm-5-series.html
ImageJ software	ImageJ	https://imagej.nih.gov/ij/plugins/colocalization.html
GraphPad Prism	GraphPad Software	https://www.graphpad.com/
Other
Sterile syringe	Shengguang	Cat#20172150535
Intravenous needles for single use (0.55 × 20 mm)	KDL	Cat#YZB/0692-2014
Surgical scissor	JZ Surgical Instruments	Cat#JC2101
Tweezer	JZ Surgical Instruments	Cat#J3C030
Sterile filter membrane (0.22 μm)	Sangon Biotech	Cat#F513163-0001
Poly-lysine coated coverslip	LYB	Cat#LY20-pll
Cell strainer	Sangon Biotech	Cat#F513441-0001
6-Well plates	Corning	Cat#3516
High precision injection pump	CNYOHO	YH42BYGH60 401A
Centrifuge	Cence	L420
Confocal microscope	Zeiss	LSM 700
Stereomicroscope	COIC	0800510
CO2 incubator	ESCO	CCL-170B-8
Clean bench	Airtech	SW-CJ-1FD