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. 2021 Jun 8;45:102037. doi: 10.1016/j.redox.2021.102037

Fig. 10.

Fig. 10

AntiOxCIN4treatment of fibroblasts from sPD patients increased cellular redox state. Reduced (A and G) and oxidized (B and F) forms of glutathione (GSH and GSSG, respectively) and nicotinamide adenine dinucleotide phosphate (NADPH and NADP+, respectively) and total cellular GSH/GSSG ratio (C) and NADPH/NADP+ ratio (H) levels were determined by a GSH/GSSG-Glo or NADP+/NADPH-Glo Assay kits, respectively, following manufacturer's instructions. mRNA levels of glutathione peroxidase 1 (GPx1) (D) and glutathione peroxidase 4 (GPx4) (E) were measured. mRNA level was normalized to the geometric mean of 4 housekeeping genes, including mitochondrial 37S ribosomal protein (MRPL51), hypoxanthine phosphoribosyltransferase 1 (HPRT1), C19orf74, family with sequence similarity 57 member A (FAM57A). Each graphics measurement corresponds to one different individual (5 fibroblasts from sPD patients and 5 fibroblasts from respective sex- and age-matched healthy controls). Data was normalized by the control condition (C = 100%). Data are expressed as mean ± SEM of 5 different experiments. Statistically significance was accepted with (*) p < 0.05, (**) p < 0.01 to C vs PD or C + AntiOxCIN4 vs PD + AntiOxCIN4 and (#) p < 0.05, (##) p < 0.01 to C vs C + AntiOxCIN4 or PD vs PD + AntiOxCIN4.