Figure 2.

Cyclin D1b is expressed in primary breast cancer. A, a TMA section consisting of normal tissue and breast cancer tissue was costained for pan-cytokeratins (green) and cyclin D1b (red) expression and visualized by immunofluorescence. Representative images of normal mammary tissue and two tumor sections with high or low D1b expression are shown. Summary of automated quantitative imaging of cyclin D1b levels within cytokeratin-positive benign or malignant epithelial cells. B, an independent tissue array containing two to six cores of invasive ductal carcinoma from 175 patients was stained for cyclin D1b expression by immunohistochemistry and scored by a pathologist. Representative images depicting the absence of staining (a) and intensity scores of 1+ (b), 2+ (c), and 3+ (d) are shown. The percentage of breast cancers exhibiting a given intensity score was quantified.