Figure 2.

Construction of DKK1 overexpression and DKK1 inference plasmids in NCL-H2170 cells. (A) Expression levels of DKK1, β-catenin and c-myc protein were detected using western blot analysis. (B) DKK1 knockdown efficiency was verified using RT-qPCR. (C) DKK1 protein and gene expression levels in co-transfected cells were detected using western blot analysis and RT-qPCR. ^*P<0.05, ^**P<0.01, ^***P<0.001. The experiments were repeated three times. CNN1, calponin 1; DKK1, Dickkopf-1; NC, negative control; OE, overexpression; RT-qPCR, reverse-transcription quantitative PCR; shRNA, short hairpin RNA.