Fig. 5.

Sequencing analysis of an extension cohort of healthy individuals and melanoma patients. A Schematic of the experimental design used in an extension cohort of plasma samples from stage IV melanoma patients and healthy individuals. B Non-metric multidimensional scaling (nMDS) of pairwise Bray-Curtis dissimilarities calculated from microbial community profiles of plasma (batches F and G) and respective DENCs at the ASV-level. C The abundance across plasma samples and DENCs of an ASV that passed all the bioinformatics decontamination criteria (with kappa = 0.667, p value < 0.001) and that was classified into the Castellaniella genus. A significant differential abundance was observed between melanoma vs. healthy control plasma samples (FDR < 0.01) based on analysis with limma