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. 2021 Jun 23;19:189. doi: 10.1186/s12951-021-00935-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — The transfection efficiency and distribution of FeSiNTs-delivered siSPAG5 in T24 bladder cancer cells. A Bright field and fluorescence microscopy images of FeSiNTs/FAM-siPAG5 fluorescence at 6 h after transfection. B Flow cytometry analysis of fluorescent cells: representative histograms (upper panel) and the mean ± SD (lower panel, from three independent experiments). FAM-siSPAG5: fluorescein-labelled small interfering RNA targeting SPAG5. C Confocal laser scanning microscopy (CLSM) analysis of the distribution of FeSiNTs/FAM-siSPAG5 in T24 bladder cancer. Fluorescein (green) labeled siSPAG5. DAPI (4ʹ,6-diamidino-2-phenylindole; blue) was used to stain cell nuclei