Fig. 3.

In vitro knockdown of SPAG5 by FeSiNTs-small interfering RNA treatment inhibits cell proliferation, migration, and invasion of T24 cells. A SPAG5 protein and mRNA levels in T24 cells upon transfection with PBS, FeSiNTs, FeSiNTs/siNC, and FeSiNTs/siSPAG5 for 48 h, analyzed by western blotting and qRT-PCR. B Effects of SPAG5 silencing by FeSiNTs/siSPAG5 on T24 proliferation, detected using MTT assays. C The migration ability of T24 cells was assessed using an in vitro scratch wound-healing assay. Images were taken 0 and 48 h after wounding of the cell monolayers. D The invasiveness of the T24 cells was evaluated using a Transwell assay, and images were captured at 48 h after incubation of the cells in a Matrigel-precoated Transwell chamber (original magnification, ×200). **P < 0.01