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. 2021 Jun 22;21:722. doi: 10.1186/s12885-021-08499-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — PC upregulates the expression of lipogenic enzymes by activating SREBP1c. A, B qRT-PCR and Western blot analysis of the mRNA and protein expression of SREBP1c in 8505C and TPC1 cells. C Results from the correlation analysis between the mRNA expression levels of PC and SREBP1c in 38 TC tissues, as shown in the scatter plot. D IHC staining was used to analyze the relationship between the protein expression of PC and SREBP1c. (E) a. Western blot analyses of SREBP1c and FASN in 8505C/PC-WT cells transfected with si-SREBP1c or control siRNA (siCtrl) and in 8505C/PC-sh cells overexpressing SREBP1c vector or empty vector (EV); the full blot is provided in supplementary file. b. Quantitative analysis of FASN protein expression. ^* p < 0.05, the si-Ctrl group compared with the si-SREBP1c and PC-sh group, the PC-sh group compared with the PC-sh + SREBp1c group. F The cellular TAG content was measured in 8505C cells. G Nile red dye and DAPI staining were used to detect the neutral lipid content in 8505C cells by microscopy with a 40× objective lens. H 8505C/PC-WT cells transfected with si-SREBP1c and 8505C/PC-sh cells overexpressing SREBP1c vector were stained with Nile red dye and subjected to flow cytometry analysis. The results are expressed as means ± standard deviation (SD) of three independent experiments and shown in histograms on the right. ^* p < 0.05; ^*** p < 0.01 versus 8505C/PC-WT cells transfected with control siRNA (siCtrl) and 8505C/PC-sh cells overexpressing empty vector (EV). Note: SREBP (sterol regulatory element-binding protein)