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. 2021 Jun 22;21:722. doi: 10.1186/s12885-021-08499-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — PC upregulates the expression of SREBP1c by activating the Akt/mTOR signaling pathway. (A) a. Western blot analysis of phosphorylated Akt (p-Akt), mTOR and phosphorylated mTOR (p-mTOR) in 8505C/PC-WT and 8505C/PC-sh cells; the full blot is provided in supplementary file. b. Quantitative analysis of the expression of each protein. ^* p < 0.05, compared with the PC-WT group. B qRT-PCR analyses of SREBP1c mRNA in different 8505C cells treated with the Akt inhibitor LY294002. (C) a. Western blot analysis of the protein expression of SREBP1c, p-Akt, and p-mTOR in different 8505C cells treated with an Akt inhibitor, LY294002; the full blot is provided in supplementary file. b. Quantitative analysis of p-mTOR protein expression. ^* p < 0.05, compared with the PC-WT group. c. Quantitative analysis of p-AKT protein expression. ^* p < 0.05, compared with the PC-WT group. D The cellular TAG content was measured in different 8505C cells. E Nile red dye and DAPI staining were used to detect the neutral lipid content in 8505C cells by microscopy with a 40× objective lens. F 8505C/PC-WT cells treated with LY294002 and 8505C/PC-sh cells treated with LY294002 were stained with Nile red dye and subjected to flow cytometry analysis. The results are expressed as means ± standard deviation (SD) of three independent experiments and shown in histograms on the right. ^* p < 0.05; ^*** p < 0.01 versus 8505C/PC-WT cells treated with vehicle and 8505C/PC-sh cells treated with vehicle. Note: mTOR (mechanistic target of rapamycin)