Table 3.

Key points of the methodology to detect pfhrp2/3 deletions by molecular analysis

First author	Method of malaria diagnosis/confirmation	N total	N cases	N pfhrp	Genes study by molecular analysis (Pfhrp2/Pfhrp3/pfhrp2/3/Flanking genes)	Method to test DNA quality	Elimination for parasitaemia ≤ 5 p/µL
Abdallah [19]	PCR	68	68	68	pfhrp2, pfhrp3, pfhrp2/3, flanking genes	PCR	NA
Okoth [39]	Microscopy/PCR	203	175	175	pfhrp2, pfhrp3, pfhrp2/3, flanking genes	PCR, qPCR	No
Akinyi [57]	PCR	188	188	188	pfhrp2, flanking genes	PCR	NA
Amoah [74]	RDT, microscopy/PCR	558	288	288	pfhrp2, pfhrp, pfhrp2/3	PCR	NA
Atroosh [48]	RDT, microscopy	622	189	189	pfhrp2	PCR	NA
Baker [15]	RDT, microscopy	458	458	458	pfhrp2, pfhrp3	NA	NA
Baldeviano [59]	Microscopy/PCR	210	54	54	pfhrp2	PCR	NA
Berhane [14]	RDT, microscopy/PCR	51	50	50	pfhrp2, pfhrp3, pfhrp2/3, flanking genes	PCR	NA
Berzosa [21]	RDT/PCR	1724	1724	122	pfhrp2, pfhrp3, pfhrp2/3	PCR	NA
Beshir [49]	RDT, microscopy/PCR	274	131	131	pfhrp2, pfhrp3, pfhrp2/3, flanking genes	qPCR	Yes
Bharti [24]	RDT, microscopy/PCR	1521	1521	50	pfhrp2, pfhrp3, pfhrp2/3, flanking genes	qPCR	No
Deme [38]	PCR	74	74	NA	NA	PCR	NA
Dong [58]	Microscopy/PCR	306	306	306	pfhrp2	PCR	NA
Dorado [20]	RDT, microscopy/PCR	374	365	365	pfhrp2, pfhrp3, pfhrp2/3, flanking genes	PCR	NA
Fontecha [41]	Microscopy/PCR	128	128	128	pfhrp2, pfhrp3, pfhrp2/3, flanking genes	PCR	NA
Funwei [69]	RDT, microscopy/PCR	511	340	66	pfhrp2, pfhrp3, pfhrp2/3	PCR	NA
Gupta [22]	RDT, microscopy/PCR	9124	1162	69	pfhrp2, pfhrp3	PCR, qPCR	Yes
Gamboa [18]	RDT, microscopy/PCR	157	157	157	pfhrp2, pfhrp3, pfhrp2/3, flanking genes	PCR	NA
Herman [43]	RDT/PCR	9317	2695	7	pfhrp2	PCR	NA
Kobayashi [52]	RDT, microscopy/PCR	5167	1189	36	pfhrp2	qPCR	Yes
Koita [28]	RDT, microscopy	723	480	37	pfhrp2	PCR	NA
Kozycki [11]	RDT, microscopy	8757	3291	370	pfhrp2	PCR	Yes
Kreidenweiss [55]	RDT/PCR	200	200	95	pfhrp2	PCR, qPCR	Yes
Kumar Bharti [71]	RDT/PCR	1392	1392	1392	pfhrp2, pfhrp3, pfhrp2/3	PCR	NA
Kumar [42]	RDT, microscopy/PCR	48	48	48	pfhrp2, pfhrp3, pfhrp2/3, flanking genes	PCR	NA
Kumar [50]	RDT/PCR	140	48	48	pfhrp2, pfhrp3	qPCR	No
Laban [54]	RDT, microscopy/PCR	3292	61	61	pfhrp2	PCR, qPCR	Yes
Li [45]	RDT/PCR	97	97	97	pfhrp2, pfhrp2/3	qPCR	No
Maltha [13]	RDT, Microscopy/PCR	182	74	74	pfhrp2, pfhrp3, pfhrp2/3	qPCR	No
Menegon [23]	Microscopy/PCR	144	144	144	pfhrp2, pfhrp3, pfhrp2/3, flanking genes	PCR	NA
Murillo Solano [63]	RDT, microscopy/PCR	115	100	100	pfhrp2, pfhrp3, pfhrp2/3, flanking genes	NA	NA
Mussa [61]	RDT/PCR	59	26	26	pfhrp2	PCR	NA
Nderu [9]	PCR	400	400	400	pfhrp2, pfhrp3	PCR	NA
Nderu [60]	RDT, microscopy/PCR	80	80	80	pfhrp2	PCR	NA
Owusu [47]	RDT, microscopy/PCR	401	62	8	pfhrp2, pfhrp3, pfhrp2/3, flanking genes	PCR	NA
Okoth [44]	Microscopy/PCR	4	4	4	pfhrp2, pfhrp3, pfhrp2/3	PCR	NA
Parr [46]	RDT, microscopy/PCR	7137	2752	2752	pfhrp2, pfhrp3, pfhrp2/3	qPCR	No
Pati [25]	RDT, Microscopy/PCR	1058	384	384	pfhrp2, pfhrp3, pfhrp2/3, flanking genes	PCR	NA
Plucinski [51]	RDT/PCR	1267	458	5	pfhrp2, pfhrp3, pfhrp2/3	qPCR	No
Rachid Viana [40]	Two RDT, Microscopy	223	223	223	pfhrp2, pfhrp3, pfhrp2/3, flanking genes	PCR	NA
Ramutton [37]	RDT/PCR	3826	77	77	pfhrp2, pfhrp3	PCR, qPCR	Yes
Ranadive [53]	RDT/PCR	1353	162	9	pfhrp2	PCR, qPCR	Yes
Sáenz [56]	Microscopy/PCR	32	32	32	pfhrp2, pfhrp3, pfhrp2/3	PCR	NA
Thomson [12]	RDT, microscopy/PCR	911	718	411	pfhrp2, pfhrp3, pfhrp2/3	PCR, qPCR	Yes
Trouvay [73]	RDT, microscopy/PCR	359	221	221	pfhrp2, pfhrp3	PCR	NA
Willie [30]	RDT/PCR	169	169	137	pfhrp2	PCR	NA
Willie [62]	RDT, microscopy/PCR	260	73	73	pfhrp2	PCR	NA
Wurtz [16]	RDT, microscopy/PCR	136	125	125	pfhrp2, pfhrp3	qPCR	No

N total total of samples included in the study, N cases total of P. falciparum confirmed cases included in the study, N pfhrp no. of samples included for molecular analysis (PCR), pfhrp2/3 double deletion pfhrp2 + pfhrp, NA not applicable