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. 2021 Jun 4;8:675179. doi: 10.3389/fmolb.2021.675179

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Copyright © 2021 Du, Nai, Shen, Li, Huang, Han, Wang, Pang and Jin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IL-21 influence the IFN-γ expression. (A,B) Percentages of IFN-γ⁺ T-cells among CD8⁺ T cells at different activation time points were evaluated by intracellular FACS assay. Results from flow plots of one representative donor and summary data for indicated CAR-T cell group were shown. (C) IFN-γ concentraction in different culture supernatants was detected by ELISA at various time points after stimulation. Each experiment was performed in triplicate, and error bars represent ± SEM. Statistical significance was tested using Students’s T-test (*p < 0.05, **p < 0.01, ***p < 0.001).