Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 4;8:675179. doi: 10.3389/fmolb.2021.675179

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Du, Nai, Shen, Li, Huang, Han, Wang, Pang and Jin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

IL-15+IL-21 is the optimal cytokine combination for the CAR-T cell preparation in vitro. (A) The absolute numbers of gated total CAR-T cells and different CAR-T subtypes were assessed on day 12 after transfection. (B) Cytotoxicity of HER-2-specific CAR-T cultured under different cytokine conditions was evaluated by Calcein AM release assay after co-culture with SK-BR-3 for 6 h at an effector to target ratio of 2:1. (C) Supernatant was collected after 24 h co-culture of HER-2 CAR-T cells with SK-BR-3 at 2:1 effector-to-target ratio and assayed for IFN-γ and granzyme B release by ELISA. Each experiment was performed in triplicate. Mean values were calculated for each group. Error bars represent ±SEM. Statistical significance was calculated using Student t test, *p < 0.05, **p < 0.01, ***p < 0.001.