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. 2021 Jun 23;19:188. doi: 10.1186/s12951-021-00934-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 9 — In vivo suppression activities of TPL/NPs on primary tumors and lung metastasis in the 4T1 xenograft model. a Growth curve of the 4T1 xenografts in BALB/c mice treated with saline, blank NPs, free TPL, TPL/NPs (L), and TPL/NPs (H) for 20 days. Data are presented as the mean ± standard error (SD, n = 6 mice). b Body weight as a function of time. c Picture of excised tumors at the end of the experiment. d Tumor weight at the end of the experiment. e Representative photograph of the 4T1 mouse lungs with the India ink-stained whole lungs at the end of the experiment. Representative tumors are marked by arrows. f Semiquantitative analysis of metastatic nodules in lungs at the end of the experiment when treated with saline, blank NPs, free TPL, TPL/NPs (L), and TPL/NPs (H) for 20 days. Note: *p < 0.05