Fig. 2.

General Features of ALPHs. A Proteomes with (orange) and without (blue) ALPHs. B Number of ALPH proteins per organism. C Sizes of the different ALPH ‘domains’ (N-terminus, catalytic domain, C-terminus) are presented as box plot (waist is median; box is IQR; whiskers are ± 1.5 IQR) and in addition as individual data points (transparent blue circles). The catalytic domain is defined as the range between the first and last motif, with an additional six N-terminal amino acids and an additional eight C-terminal amino acids (compare F). D Distances between the six different motifs (in amino acids) are presented as box plot (waist is median; box is IQR; whiskers are ± 1.5 IQR) and in addition as individual data points (transparent blue circles). E Sequence motifs were created with WebLogo [31]. The most obvious differences between the three groups are marked with orange bars. F The sequence of ALPH from S. cerevisiae is shown as a typical example, to illustrate the position of the catalytic domain (blue), the N- and C-termini (grey) as well as all six motifs (red) and the distances between the motifs that we found most conserved (green). Details on all data presented in Fig. 2 can be found in Additional file 3: Table S1