Effective bending modulus, keff, as a function of the gel phase area fraction, ϕ, measured by NSE (points) and calculated from the gel and fluid phase moduli (lines). keff was calculated using the averaged values of kgel = 202 kBT and kfluid = 28 kBT according to a weighted harmonic average (eqn (3), dotted line, χ2 = 18.7) and theory by Netz and Pincus (eqn (4), solid line, χ2 = 11.8) described in the text. Values in the legend refer to the membrane composition.