Prevalence of positive SPTs to Alternaria, Aspergillus, and Cladosporium according to age distribution (0–20, 21–40, 41–60, and 61–80 years) during the 2 decades investigated. Total patient numbers for the 2 decades 1998–2007 versus 2008–2017: Alternaria, n = 1,845 patients tested in 1998–2007 and n = 1,335 in 2008–2017; Aspergillus, n = 1,995 and n = 1,352 patients; Cladosporium, n = 1,408 patients and n = 1,037 patients. Significance levels reflect comparisons within each age-group between the first and second decades for each allergen. Significance levels: **p < 0.01, ***p < 0.001. SPT, skin prick test.