Fig. 2.

Characterization of purified NbSBT1 and NbSBT2. Hexahistidine‐tagged NbSBT1 and NbSBT2 produced in N. benthamiana were purified by metal‐chelate affinity chromatography and then analysed by SDS/PAGE under reducing conditions followed by staining with CBB. For ABPP, purified NbSBTs were incubated with 100 µm FP‐biotin (NbSBT1) or 10 μm biotinyl‐YVAD‐CMK (NbSBT2) for 1 h at 37 °C prior to analysis by SDS/PAGE under reducing conditions and western blotting using streptavidin–peroxidase for detection. The migration positions of selected molecular mass standards are indicated, with their respective masses expressed in kDa.