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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Nat Protoc. 2021 Mar 10;16(4):1936–1965. doi: 10.1038/s41596-020-00474-1
Poring pulse Transfer pulse

Voltage 200 V 20 V
Pulse length 5 ms 50 ms
Pulse interval 50 ms 50 ms
Number of pulses 2 5
Decay rate 10% 40%
Polarity + +/−