Extended Data Fig. 7 |. ELISA of wild-type or mutant RBD for monoclonal antibodies.

a, EC₅₀ values for binding to wild-type RBD of shared singlets and shared clones of monoclonal antibodies obtained at the initial 1.3- and 6.2-month follow-up visit, divided by participant (n = 6 (COV21), n = 13 (COV47), n = 3 (COV57), n = 6 (COV72), n = 15 (COV96), n = 9 (COV107)). Lines connect shared singlets or clones. Monoclonal antibodies with improved EC₅₀ at the 6.2-month follow-up visit are highlighted in green; remaining monoclonal antibodies are shown in black. Statistical significance was determined using two-tailed Wilcoxon matched-pairs signed-rank test. b–j, Graphs show ELISA binding curves for different antibodies obtained at 1.3 months (dashed lines) and their clonal relatives obtained after 6.2 months (solid lines) binding to wild type, R346S, E484K, Q493R, N439K, N440K, A475V, S477N, V483A and V367F RBDs (colours as indicated). Antibody identifiers of pairs are as indicated on top of panels (1.3 months/6.2 months). k, Heat map shows log₂-transformed relative fold change in EC₅₀ against the indicated RBD mutants for 52 antibody clonal pairs obtained at 1.3 (black) and 6.2 months (red). The clonal and participant origin for each antibody pair is indicated above.