a, Global viSNE projection of pooled B cells for all participants pooled shown in background contour plots, with overlaid projections of concatenated controls, and convalescent participants at 1.3 and 6.2 months. b, viSNE projection of pooled B cells for all participants of B cell clusters identified by FlowSOM clustering. Column-scaled z-scores of median fluorescence intensity (MFI) as indicated by cluster and marker. c, Frequency of B cells from each group in FlowSOM clusters indicated. Each circle represents an individual control individual (n = 20) (grey), convalescent participant at 1.3-month post-infection (n = 41) (red) or convalescent participant at 6.2 months post-infection (n = 41) (green). Horizontal bars indicate mean values. Significance determined by two-tailed paired t-test for comparisons between time points within individuals and two-tailed unpaired t-test for comparison between controls and convalescent individuals. d, Individual viSNE projections of indicated protein expression.