Figure 1.

The overview of single-cell omics techniques. (A) The overview of single-cell cytometry systems, including flow cytometry with fluorescence-labeled antibodies for single isolated cells (left), mass cytometry with metal isotope-conjugated antibodies isolated single cells (middle), and imaging mass cytometry with metal isotope-conjugated antibodies labeled on tissues (right). (B) The overview of two canonical scRNA-seq platforms, including plate-based scRNA-seq methods with sorted cells barcoded within each well (left), and droplet-based scRNA-seq method, single cells were barcoded within individual droplets (right). (C) The overview of single-cell multi-omics techniques, including library preparing for genomic, epigenomic, proteomic, and spatial indexing with transcriptomic of single cells simultaneously.