Figure 2.

Maturation of cDC1, cDC2, and CD14⁺ DCs. DCs were inoculated with PRRSV1 3267 (MOI 10), live or UV-inactivated (UV3267), for 6 h prior to (for example, 3267+Poly I:C) or simultaneously with (for example, 3267/Poly I:C) poly I:C (10 μg/ml, to cDC1, cDC2, and CD14⁺ DCs) or gardiquimod (10 μg/ml, to only cDC2 and CD14⁺ DCs). Plain medium (complete RPMI 1640) was used as the negative control (mock) in each step. After incubation for extra 18 h, cells were harvested and stained for MHC-I, MHC-II, CD80/CD86, and CCR7 on cDC1 (A), cDC2 (B), and CD14⁺ DCs (C). Data of cDC1 were obtained from two pigs, while cDC2 and CD14⁺ DCs were from four pigs. The histograms shown are from one pig as the representative.