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. 2021 Jun 2;12:674185. doi: 10.3389/fimmu.2021.674185

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Copyright © 2021 Li and Mateu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Response of cDC2 and CD14⁺ DCs to different doses of PRRSV1 3267 infected MARC-145 cells. (A) An increasing % of infected (PRRSV1 N⁺) MARC-145 cells (black bars) was generated by infecting MARC-145 cells with different doses of MARC3267. The viability was indicated upper (orange line); (B) Expression of MHC-I, MHC-II, CD80/86, and CCR7 in cultures of cDC2 (upper) and CD14⁺ DCs (lower) exposed to MARC-145 cells containing different % of infected cells (indicated at the left of Y-axis); (C) Cytokine production in cultures of cDC2 (upper) and CD14⁺ DCs (lower) exposed to MARC-145 cells containing an increasing % of infected cells (indicated below IL-12/IL-23p40 of CD14⁺ DCs). IL-10 was not detected in cDC2, thus not shown.