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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: FASEB J. 2021 Jul;35(7):e21732. doi: 10.1096/fj.202100385R

Figure 3.

Figure 3.

mTOR-inhibition prevented phenotype switch in the Asah1fl/fl/SMCre mice. Representative images from the aortic sections depicts changes in the expression of (A) SM22‐α (brown stain) (B) OSP and (C) RUNX2 in the aortic media of Asah1fl/fl/SMCre mice. Summarized bar graph showed Torin-1 significantly increased (D) SM22‐α and decreased (E) OSP (F) RUNX2 in aortic medial wall of mice receiving Vit D injections. n=5, SMC: smooth muscle cell; Vehl: vehicle; Vit D: vitamin D; Tor: Torin-1; OSP, osteopontin; RUNX2, runt‐related transcription factor 2, ‘n’ is mouse number. *P < 0.05 vs WT/WT Vehl; #P < 0.05 vs WT/WT Vit D group; $P< 0.05 vs. Asah1fl/fl/SMCre Vit D group by one-way ANOVA with Holm-Sidak’s test post-hoc analysis. Data are shown as mean ± SEM of values.