Fig. 2. The general workflow of ABPP using ABPs and AfBPs, from identification to subsequent enrichment and quantification of target proteins. Target identification using ABPs without (1) and with (2) a bioorthogonal reporter. Target identification using AfBPs without (3) and with (4) a bioorthogonal reporter. ABP-modified proteins can be detected and identified using a variety of biochemical and cell biological techniques including gel-based fluorescence analysis, mass spectrometry-based analysis and bioimaging.
