Fig. 3. Kinase inhibitor-based ABPs and AfBPs and their in cellulo applications. (A) Reported kinase inhibitor-based ABPs and AfBPs. (B) Gel-based labeling of BTK with 2 in BTK-positive (Toledo, Namalwa) and BTK-negative cells (Jurkat), only positive cells showed a band around 75 kDa which demonstrated the successful labeling of cellular BTK. Reproduced from ref. 41 with permission from Royal Society of Chemistry, copyright 2019. (C) Imaging of JNK1-GFP-transfected HeLa cells using 7a with two TP reporters, both of which successfully labeled JNK1 in living cells. Reproduced from ref. 48 with permission from Royal Society of Chemistry, copyright 2019. (D) Reported sulfonyl fluoride-based ABPs for kinases (left). In-gel fluorescence analysis of Jurkat cells treated with either DMSO or the probe competitor, followed by treatment with 11a to 11c. 11 was successfully used for broad-spectrum kinase labeling in cells. P = probe, C = competitor. Reproduced from ref. 53 with permission from American Chemical Society, copyright 2017.