Fig. 4. Impact of osmolytes on non-cryopreserved A549 cell monolayers. A) Growth rates during incubation with F-12K or osmolyte for 6 days. *P < 0.0002 from control. B) Growth rates after incubation with osmolyte for 3 days, then replacing media with F-12K media for 3 days (hence data starts on day 4). *P < 0.02 from control. Shaded area represents standard error of 3 independent experiments. Plate maps show experimental design, where cells are seeded, what treatment they received, and the day when cells are removed (for counting) indicated.