Fig. 2.

Trypsinogen processing precedes LC-3 processing. a Percentage of trypsin co-localization with LC3 shows significance after 4 h and later in disease development with a maximum at 12 h after induction of pancreatitis. Data are derived from 4 independent experiment and 5 random view fields per animal. Percentage of co-localization is shown as Mender overlap coefficient value calculated using JACoP plug-in in ImageJ. b Similar kinetic was found for LAMP2 co-localization with LC3. Earliest trypsin co-localization with autophagosome markers (LC3) can be detected after 4 h. At this timepoint, also autophagosomal and lysosomal markers are co-localized (a, b). (Bar 20 µM)