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. 2021 Jun 9;13(11):14910–14923. doi: 10.18632/aging.203120

Figure 5.

Figure 5

Changes in collagen in AMC and Coleman fat after grafting. (A, B) Masson’s trichrome staining of grafts in AMC and Coleman fat (control) on Day 90 after grafting. (C–L) Immunohistochemical staining of mouse type I collagen in grafts of AMC and Coleman fat (control) on Days 7, 14, 30, 60 and 90. Red line: type I collagen thickness, from the outermost to the innermost of grafts. (M) Stiffness analysis of grafts of AMC and Coleman fat (control) on Days 7, 14, 30, 60, and 90 after grafting. (N) Collagen percentage in grafts of AMC and Coleman fat (control) on Days 7, 14, 30, 60, and 90, calculated by quantification of positive area in Masson stained sections. (O) Quantification of the area positive for mouse collagen I immunohistochemical staining in grafts of AMC and Coleman fat (control) on Days 7, 14, 30, 60 and 90. Results are presented as the mean ± SD (n = 7 per group). * P < 0.05, control vs. AMC; **, control vs. AMC; P < 0.01.