Genes |
Forward |
Reverse |
Sirt1 (m) |
5ʹ-CACTGTAACTGGGGGCAACT-3’ |
5ʹ-CACTTCTTGTCAGCGTCGAA-3’ |
Cxcl-2(m) |
5ʹ-TGTCCCTCAACGGAAGAACC-3’ |
5ʹ-CTCAGACAGCGAGGCACATC-3’ |
Il-1β(m) |
5ʹ-GCAACTGTTCCTGAACTCAACT-3’ |
5ʹ-ATCTTTTGGGGTCCGTCAACT-3’ |
Tnf-α(m) |
5ʹ-GACGTGGAACTGGCAGAAGAG-3’ |
5ʹ-TTGGTGGTTTGTGAGTGTGAG-3’ |
β-actin(m) |
5ʹ-AGTGTGACGTTGACATCCGT-3’ |
5ʹ-GCAGCTCAGTAACAGTCCGC-3’ |
LysMCre-WT(m) |
5ʹ-TTACAGTCGGCCAGGCTGAC-3’ |
LysMCre-Mutant(m) |
5ʹ-CCCAG AAATGCCAGATTACG-3’ |
|
LysMCre-Common(m) |
5ʹ-CTTGGGCTGCCAGAATTTCTC-3’ |
Sirt1 gene deletion(m) |
oIMR7909: 5ʹ-GGTTGACTTAGGTCTTGTCTG-3’ |
|
oIMR7912: 5ʹ-CGTCCCTTGTAATGTTTCCC-3’ |