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| Antibody target | Identifier | AR (pH value) | Dilution | Staining position | Opal dye |
| Ki67 | Dianova, Clone: Ki-67P, Cat#: DIA-670-P1 | 9.0 | 1:200 | 1 | 520 |
| CD8 | Oncodianova, Clone: TC8, Cat#: DIA-TC8 | 9.0 | 1:200 | 2 | 690 |
(AR = antigen retrieval, RTU = Ready to use).
