Table I.

Studies examining modulation of Wnt signaling and Tcf1.

T subsets	Experimental Models	Perturbations	Findings	Ref
Murine Th1	In vitro	Tcf7 germline KO	Tcf1 represses IFNγ	25
Murine Th1/Tfh	LCMV	Tcf7 long isoforms KO	Tcf1 required for T memory formation	9
Murine Th17	In vitro, EAE	Tcf7 germline KO	Tcf1 represses IL17a	28, 29
Murine Th17	Colitis, colorectal cancer	Constitutively active β-catenin	Tcf1 binds Rorc locus in chromatin accessible regions	30
Murine Thymocytes	T cell development	Tcf7 germline KO, constitutively active β-catenin	Tcf1 promotes thymocyte survival via enhancement of Rorγt	31
Human Th17	In vitro	Wnt inhibitors	Enhanced Th17 differentiation	32
Human Th17	In vitro	Wnt3a, Wnt5a stimulation	Reduced Th17 differentiation	32
Murine Tregs	In vivo T cell development	Tcf7 hemizygous	Higher Treg percentage	44
Human and murine Tregs	In vitro	None	Tcf1 interacts with and inhibits Foxp3-mediated transcription	45
Human and murine Tregs	In vitro	Gsk3β inhibitor, Wnt3a stimulation	Reduced Treg suppressive capacity	45
Murine Tregs	In vivo steady state	Constitutively active β-catenin	Spontaneous colitis	30
Murine Tregs	In vivo steady state	Treg-specific Tcf1/Lef1 double KO	Spontaneous multi-organ autoimmunity	46,47