Table 1. Characteristics of the included studies.

Author	Year	County	Sample type	Reference	Diagnosis	Target gene	PCR method	N	TP	FP	FN	TN	Type of research	Sample condition	Patient selection method
Che, N.	2017	China	Pleural effusion	CRS	Tuberculosis pleurisy	IS6110	Real-Time PCR	78	45	0	15	18	prospective	Frozen	Consecutive
Yang, J.a	2017	China	Plasma	CRS	Pulmonary TB	IS6110	Digital PCR	56	28	0	0	28	Case-control	Frozen	Convenience
Yang, J.b	2017	China	Plasma	CRS	Extrapulmonary TB	IS6110	Digital PCR	56	28	0	0	28	Case-control	Frozen	Convenience
Yang, J.c	2017	China	Plasma	CRS	Pulmonary TB	IS6110	Real-Time PCR	56	14	0	14	28	Case-control	Frozen	Convenience
Yang, J.d	2017	China	Plasma	CRS	Extrapulmonary TB	IS6110	Real-Time PCR	56	15	0	13	28	Case-control	Frozen	Convenience
Shou, J.	2018	China	Pleural effusion	CRS	Tuberculosis pleurisy	Unreported	Real-Time PCR	136	65	0	38	33	prospective	Fresh	Consecutive
Li, X.	2020	China	Cerebrospinal fluid	CRS	Tuberculosis meningitis	IS6110	Real-Time PCR	68	26	0	20	22	prospective	Fresh	Consecutive
Lyu, L.a	2020	China	Plasma	CRS	Pulmonary and extrapulmonary TB	IS6110	Digital PCR	261	63	7	92	99	Case-control	Frozen	Consecutive
Lyu, L.b	2020	China	Plasma	CRS	Pulmonary and extrapulmonary TB	IS1081	Digital PCR	261	42	7	113	99	Case-control	Frozen	Consecutive
Shao, L.	2020	China	Cerebrospinal fluid	CRS	Tuberculosis meningitis	IS6110	Real-Time PCR	84	32	0	28	24	prospective	Frozen	Consecutive
Sharma, P.	2020	India	Ascitic fluid	CRS	Abdominal tuberculosis	devR	Real-Time PCR	65	22	1	33	9	prospective	Frozen	Consecutive
Yang, X.	2020	China	Pleural effusion	CRS	Tuberculosis pleurisy	IS6110	Real-Time PCR	286	153	0	68	65	prospective	Frozen	Consecutive
Han, B.a	2020	China	Plasma	CRS	Pulmonary TB	Unreported	Real-Time PCR	140	63	3	16	58	Case-control	Frozen	Convenience
Han, B.b	2020	China	Plasma	CRS	Extrapulmonary TB	Unreported	Real-Time PCR	100	31	3	8	58	Case-control	Frozen	Convenience
Ushio, R.a	2016	Japan	Plasma	Culture	Pulmonary TB	IS6110	Digital PCR	52	21	1	12	18	Case-control	Frozen	Convenience
Ushio, R.b	2016	Japan	Plasma	Culture	Pulmonary TB	gyrB	Digital PCR	52	10	0	23	19	Case-control	Frozen	Convenience
Labugger, I.	2017	Germany	Urine	Culture	Pulmonary TB	IS6110	Real-Time PCR	19	7	0	4	8	Prospective	Frozen	Consecutive
Patel, K.	2018	South Africa	Urine	Culture	Pulmonary TB	DR region	Real-Time PCR	412	75	27	100	210	Cohort study	Frozen	Convenience

PCR, polymerase chain reaction; TP, true positive; FP, false positive; FN, false negative; TN, true negative; CRS, composite reference standard; TB, tuberculosis; DR, direct repeat.