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. 2021 Apr 30;129(1):98–113. doi: 10.1161/CIRCRESAHA.120.318402

Figure 7.

Figure 7.

Diabetes-induced arrhythmogenic action potential remodeling is dependent predominantly on CaMKIIδ (Ca2+/calmodulin-dependent protein kinase II)-S280 O-GlcNAcylation. A, Action potential duration (APD) prolongation and alternans in streptozotocin (STZ)-treated wild-type (WT) murine ventricular myocytes (n=16 cells from 7 animals). B, Arrhythmogenic action potential (AP) remodeling in STZ was abolished by AIP (autocamtide-2-related inhibitory peptide; n=11 cells from 4 animals). C, APD prolongation was prevented in STZ-treated CaMKIIδ-S280A (n=22 cells from 6 animals). D, APD prolongation and alternans in STZ-treated CaMKIIδ-mutated Met281Val and Met282Val (MMVV; n=17 cells from 6 animals). Nested t test. E and F, AP remodeling was CaMKII-dependent and involved both hyperglycemia-dependent S280 O-GlcNAcylation (predominant mechanism) and Ang II-dependent 281/2MM oxidation (n=total number of cells/animals is reported in the figure). Nested 1 way ANOVA, followed by Dunnett multiple comparisons test. CaSpF indicates Ca2+ spark frequency.