Figure 6.

AHNAK dictates the choice between apoptosis and senescence in transformed and non-transformed cells

(A) BJ and U2OS cells transfected with the indicated siRNAs and treated with either DMSO or Nutlin-3 (20 μM). Apoptosis was assayed using FACS with antibody to annexin V-Alexa 488.

(B) Percentage of annexin-V-positive cells in BJ.

(C) Percentage of annexin-V-positive cells in U2OS.

(D) Representative bright-field images of SA-β-gal staining of DMSO or Nutlin-3-treated BJ fibroblast transfected with indicated siRNAs.

(E) Graphical representation of percentage of β-gal-positive cells from (D).