Table 3.
SD-induced changes in ribosome-associated transcript abundance in PV::RiboTag mice
| Gene name | Region | Sleep, ΔCT |
SD, ΔCT |
qPCR p value (sleep vs SD) |
n (sleep) |
n (SD) |
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 3 h | 6 h | 3 h | 6 h | 3 h | 6 h | 3 h | 6 h | 3 h | 6 h | ||
| Arc | CTX | 6.64 ± 0.23 | 6.87 ± 0.07 | 5.25 ± 0.43 | 5.40 ± 0.07 | 0.0002 | <0.0001 | 4 | 5 | 4 | 6 |
| HP | 6.67 ± 0.09 | 5.46 ± 0.14 | 6.58 ± 0.11 | 5.29 ± 0.19 | 0.9758 | 0.8837 | 4 | 6 | 5 | 6 | |
| Homer1a | CTX | 7.49 ± 0.08 | 6.71 ± 0.13 | 7.03 ± 0.10 | 5.27 ± 0.12 | 0.3025 | <0.0001 | 4 | 5 | 4 | 6 |
| HP | 9.02 ± 0.23 | 7.32 ± 0.35 | 9.00 ± 0.23 | 7.04 ± 0.27 | 0.9758 | 0.8673 | 4 | 6 | 5 | 6 | |
| Narp | CTX | 8.22 ± 0.08 | 7.39 ± 0.07 | 7.77 ± 0.13 | 7.01 ± 0.09 | 0.3025 | 0.0196 | 4 | 5 | 4 | 6 |
| HP | 8.60 ± 0.15 | 8.58 ± 0.47 | 8.71 ± 0.19 | 8.22 ± 0.21 | 0.9758 | 0.8383 | 4 | 6 | 5 | 6 | |
| Bdnf | CTX | 8.03 ± 0.12 | 7.36 ± 0.19 | 7.89 ± 0.14 | 6.79 ± 0.09 | 0.6257 | 0.0014 | 4 | 5 | 4 | 6 |
| HP | 0.60 ± 0.21 | 5.38 ± 0.26 | 1.17 ± 0.27 | 5.44 ± 0.20 | 0.2158 | 0.8907 | 4 | 6 | 5 | 6 | |
| Npas4 | CTX | 9.14 ± 0.43 | 7.97 ± 0.13 | 7.96 ± 0.14 | 7.11 ± 0.10 | 0.0143 | 0.0008 | 4 | 5 | 4 | 6 |
| HP | 9.15 ± 0.20 | 5.21 ± 0.31 | 9.36 ± 0.13 | 5.40 ± 0.12 | 0.2737 | 0.6749 | 4 | 6 | 5 | 6 | |
| cFos | CTX | 7.81 ± 0.10 | 7.04 ± 0.13 | 6.85 ± 0.38 | 5.21 ± 0.13 | 0.0336 | <0.0001 | 4 | 5 | 4 | 6 |
| HP | 9.81 ± 0.13 | 7.56 ± 0.32 | 9.51 ± 0.10 | 5.98 ± 0.18 | 0.2081 | 0.0042 | 4 | 6 | 5 | 6 | |
| FosB | CTX | 7.93 ± 0.16 | 7.05 ± 0.17 | 7.68 ± 0.15 | 6.22 ± 0.20 | 0.5072 | 0.0008 | 4 | 5 | 4 | 6 |
| HP | 10.86 ± 0.12 | 6.65 ± 0.45 | 10.42 ± 0.11 | 7.10 ± 0.40 | 0.0814 | 0.5437 | 4 | 6 | 5 | 6 | |
| Clock | CTX | 3.58 ± 0.07 | 3.29 ± 0.09 | 3.47 ± 0.04 | 3.04 ± 0.06 | 0.8513 | 0.2076 | 4 | 5 | 4 | 6 |
| HP | 4.11 ± 0.05 | 4.06 ± 0.58 | 4.02 ± 0.05 | 2.68 ± 0.08 | 0.9716 | 0.9770 | 4 | 6 | 5 | 6 | |
| Bmal1 | CTX | 5.42 ± 0.09 | 4.51 ± 0.08 | 5.25 ± 0.07 | 4.51 ± 0.04 | 0.6893 | 0.9848 | 4 | 5 | 4 | 6 |
| HP | 5.86 ± 0.09 | 3.71 ± 0.56 | 5.93 ± 0.09 | 5.59 ± 0.12 | 0.9716 | 0.9770 | 4 | 6 | 5 | 6 | |
| Cry1 | CTX | 8.57 ± 0.08 | 4.45 ± 0.03 | 8.63 ± 0.13 | 4.51 ± 0.05 | 0.8942 | 0.9436 | 4 | 5 | 4 | 6 |
| HP | 6.24 ± 0.04 | 3.98 ± 0.60 | 6.15 ± 0.07 | 4.22 ± 0.23 | 0.9716 | 0.9099 | 4 | 6 | 5 | 6 | |
| Cry2 | CTX | 6.37 ± 0.05 | 3.12 ± 0.07 | 6.42 ± 0.09 | 3.09 ± 0.06 | 0.8942 | 0.979 | 4 | 5 | 4 | 6 |
| HP | 5.18 ± 0.05 | 3.63 ± 0.61 | 5.09 ± 0.04 | 2.82 ± 0.07 | 0.9716 | 0.9994 | 4 | 6 | 5 | 6 | |
| Per1 | CTX | 4.16 ± 0.12 | 2.61 ± 0.10 | 3.92 ± 0.09 | 2.45 ± 0.04 | 0.4605 | 0.5214 | 4 | 5 | 4 | 6 |
| HP | 4.66 ± 0.11 | 3.57 ± 0.53 | 4.56 ± 0.05 | 2.17 ± 0.08 | 0.9716 | 0.9994 | 4 | 6 | 5 | 6 | |
| Per2 | CTX | 7.70 ± 0.23 | 6.34 ± 0.15 | 7.21 ± 0.03 | 5.95 ± 0.15 | 0.0121 | 0.0113 | 4 | 5 | 4 | 6 |
| HP | 6.98 ± 0.23 | 4.46 ± 0.89 | 6.88 ± 0.11 | 5.05 ± 0.24 | 0.9716 | 0.6197 | 4 | 6 | 5 | 6 | |
| Rev-Erba | CTX | 3.89 ± 0.03 | 2.51 ± 0.06 | 3.91 ± 0.02 | 2.47 ± 0.07 | 0.9536 | 0.9276 | 4 | 5 | 4 | 6 |
| HP | 4.97 ± 0.07 | 2.34 ± 0.09 | 4.96 ± 0.06 | 2.26 ± 0.11 | 0.9993 | 0.9805 | 4 | 6 | 5 | 6 | |
| Dbp | CTX | 6.42 ± 0.07 | 5.47 ± 0.10 | 6.35 ± 0.09 | 5.49 ± 0.07 | 0.7058 | 0.9276 | 4 | 5 | 4 | 6 |
| HP | 7.62 ± 0.08 | 6.12 ± 0.23 | 7.63 ± 0.10 | 6.10 ± 0.25 | 0.9993 | 0.9805 | 4 | 6 | 5 | 6 | |
| Tef | CTX | 3.98 ± 0.02 | 3.35 ± 0.06 | 3.96 ± 0.02 | 3.24 ± 0.05 | 0.9536 | 0.7245 | 4 | 5 | 4 | 6 |
| HP | 4.80 ± 0.09 | 3.23 ± 0.05 | 4.80 ± 0.02 | 2.94 ± 0.07 | 0.9993 | 0.7872 | 4 | 6 | 5 | 6 | |
| Nfil3 | CTX | 7.11 ± 0.05 | 6.46 ± 0.17 | 6.88 ± 0.06 | 6.13 ± 0.07 | 0.0102 | 0.0206 | 4 | 5 | 4 | 6 |
| HP | 7.86 ± 0.10 | 8.58 ± 0.47 | 7.71 ± 0.13 | 8.22 ± 0.21 | 0.6950 | 0.713 | 4 | 6 | 5 | 6 | |
| Dec1 | CTX | 4.88 ± 0.04 | 3.62 ± 0.09 | 4.66 ± 0.02 | 3.27 ± 0.04 | 0.0102 | 0.0143 | 4 | 5 | 4 | 6 |
| HP | 5.54 ± 0.11 | 3.92 ± 0.11 | 5.49 ± 0.07 | 3.82 ± 0.07 | 0.9951 | 0.9805 | 4 | 6 | 5 | 6 | |