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. 2021 Jun 9;12:662594. doi: 10.3389/fimmu.2021.662594

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Copyright © 2021 Berzaghi, Tornaas, Lode, Hellevik and Martinez-Zubiaurre

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of CAF-CM on DC phenotype. Gross morphology of DC cultures by phase-contrast microscopy. Immature DCs show a classical round morphology whereas mature DCs present characteristic dendrites. Incubations with CAF-CM partially revert the phenotype of mDCs into iDCs (top-right panel), whereas this effect is lost in the conditioned medium from CAFs exposed to 3x6 Gy. Cells presenting dendrites in control mDCs and fractionated radiation (3x6 Gy) CAF-CM groups are shown by arrowheads. Scale Bars = 10 μm.