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. 2021 Jun 9;12:662594. doi: 10.3389/fimmu.2021.662594

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Copyright © 2021 Berzaghi, Tornaas, Lode, Hellevik and Martinez-Zubiaurre

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CAF-induced cytokine secretion by DCs. Monocyte-derived dendritic cells in non-stimulated or stimulated conditions were incubated for 48h with irradiated or non-irradiated CAF conditioned medium (CAF-CM, left panels) or in co-cultures (CAF-CC, right panels). Resulting levels of IL-10 (top panels) and IL-12 (lower panels) found in supernatants were quantified by ELISA assays. Data represent the mean ( ± SD) values from 4 different CAF donors measured in duplicates. Brown-Forsythe and Welch ANOVA test and-values were determined between mDCs and non-irradiated CAF-CM, mDCs vs irradiated CAFs. *p ≤ 0.05, ****p ≤ 0.0001.