Figure 6.

Alterations on STAT3 signaling and NF-κB activation in DCs exposed to CAF-CM. (A) Western blot analysis, using anti-STAT3, p-STAT3 (S727), p-STAT3 (Y705), NF-κB/p65, and p-NF-κB/p65 (S536) on whole DC cell lysates stimulated with irradiated and non-irradiated CAF-CM. Results were normalized against GAPDH expression and the results of phosphorylated proteins were normalized against the respective total proteins. In (B), the relative intensity of the bands corresponding to (A), determined by densitometry, is shown as a bar graph. Data represent mean (± SD) values from 3 different CAF donors. Two-way ANOVA test and p-values were determined between non-irradiated CAFs, mDCs, and the two irradiated CAF-groups individually. *p ≤ 0.05, **p ≤ 0.01.