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. 2021 Jun 23;7(26):eabg8139. doi: 10.1126/sciadv.abg8139

Fig. 2. PC- and AC-secreted factors increase the extravasation potential of TCs.

Fig. 2

(A) Schematic of the collection of PC and AC CM and subsequent treatment of the MVNs before TC perfusion. (B) Extravasation efficiencies of breast MDA-231 in monoculture MVNs with or without CM treatment at 24 hours after perfusion. (C) Representative images of breast MDA-231 in monoculture MVNs with or without CM treatment at 24 hours after perfusion. (D) Heatmap for cytokine array showing relative magnitudes of secreted factors from MDA-231, iPS-EC, PC, and AC alone, as well as iPS-EC, PC, and AC cultured in Transwell inserts with MDA-231 cells after 24 hours of culture. (E) Relative magnitude of significantly up-regulated cytokines/chemokines by PC or AC cultured in inserts with MDA-231 compared to iPS-EC cultured with MDA-231.(F) Representative images of 2D immunofluorescence staining for F-actin + DAPI, CCL2, and PDGF-BB in iPS-ECs, PCs, and ACs, cultured with MDA-231 in Transwell inserts. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001; not significant; n.s.#, not significant across all conditions (pairwise).