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. 2021 Jun 8;10:e64901. doi: 10.7554/eLife.64901

Key resources table.

Reagent type
(species) or resource		 Designation Source or reference Identifiers Additional
information
Strain, strain background (Schizosaccharomyces pombe) Wild-type S. pombe Other FC15,
h- wild-type strain 972		 Figures 1, 2, 3, 4, 5 and 6; Figure 1—figure supplement 1, Figure 2—figure supplement 1,
Figure 2—figure supplement 2, Figure 2—figure supplement 3, Figure 5—figure supplement 1, Figure 6—figure supplement 1
FC lab collection; https://www.uniprot.org/taxonomy/284812
Genetic reagent
(S. pombe)		 cdc25-22 mutant Other FC342,
h-cdc25-22 		Figure 3;
FC lab collection; https://www.pombase.org/genotype/cdc25-22-C532Y-amino_acid_mutation-expression-not_assayed
Genetic reagent
(S. pombe)		 cut7-446 mutant Other FC1455,
h-cut7-446 leu1-32 		Figure 4;
FC lab collection; https://www.pombase.org/genotype/cut7-446-I954T-amino_acid_mutation-expression-knockdown
Genetic reagent
(S. pombe)		 cdc16-116 mutant Other FC13,
h-cdc16-116 		Figure 4;
FC lab collection; https://www.pombase.org/genotype/cdc16-116-unknown-unknown-expression-not_assayed
Genetic reagent
(S. pombe)		 mid2 mutant Other FC881
h-mid2::kanMX ade6 leu1-32
ura4-D18 		Figure 7;
FC lab collection; https://www.pombase.org/genotype/mid2delta
Genetic reagent
(S. pombe)		 gpd1 mutant Other FC3291,
h-gpd1::hphMX6ade6-M216leu1-32 ura4-D18his3-D1 		Figure 6—figure supplement 2
FC lab collection
Peptide, recombinant protein Bovine Serum Albumin Sigma Aldrich Cat. #: A3608
Peptide, recombinant protein Lectin Sigma Aldrich Cat. #: L1395
Peptide, recombinant protein RNAse Thermo Scientific Cat. #: EN0531
Chemical compound, drug Latrunculin A Abacam Cat. #: ab144290
Chemical compound, drug BODIPY 493/503 Thermo Fisher Cat. #: D3922
Chemical compound, drug FITC Sigma Aldrich Cat. #: F7250
Chemical compound, drug OptiPrep Sigma Aldrich Cat. #: D1556
Software, algorithm Matlab Mathworks R2019a
Software, algorithm FIJI https://imagej.net/Fiji/Downloads v. 1.53c
Software, algorithm Algorithm to retrieve phase information https://bitbucket.org/kchuanglab/quantitative-phase-imaging/src/master/ Bostan et al., 2016
Software, algorithm Morphometrics SimTK: Morphometrics: Project Home Ursell et al., 2017
Other CellASIC Onix2 microfluidic control system Merck Cat. #:
CAX2-S0000
Other CellASIC ONIX microfluidic plates Merck Cat. #: Y04C-02-5PK
Other Optical filter Chroma Technology Cat. #: D680/3m