Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens, female) | HEK293 | ATCC | Cat. #: ATCC-CRL-1573 RRID:CVCL_0045 |
Lot/Batch No: 63777489 |
| Recombinant DNA reagent | pcDNA3.1 GA65-GFP | This paper | Described in Results part 1 and Materials and methods section | |
| Recombinant DNA reagent | pcDNA3.1 NES-GA65-GFP | This paper | Described in Results part 1 and Materials and methods section | |
| Recombinant DNA reagent | pcDNA3.1 NLS-GA65-GFP | This paper | Described in Results part 1 and Materials and methods section | |
| Recombinant DNA reagent | pcDNA3.1 NLS-GA65-GFP (K7T, K15T) | This paper | Described in Results part 1 and Materials and methods section | |
| Recombinant DNA reagent | pcDNA3.1 GA65-GFP-PY | This paper | Described in Results part 1 and Materials and methods section | |
| Recombinant DNA reagent | pcDNA3.1 GA65-GFP-PY(P410L) | This paper | Described in Results part 1 and Materials and methods section | |
| Recombinant DNA reagent | pcDNA3.1 GFP | This paper | Described in Results part 1 and Materials and methods section | |
| Recombinant DNA reagent | pcDNA3.1 PR73-GFP | This paper | Described in Results part 3 and Materials and methods section | |
| Recombinant DNA reagent | pcDNA3.1 GR73-GFP | This paper | Described in Results part 3 and Materials and methods section | |
| Recombinant DNA reagent | pcDNA3.1 (G4C2)73-GFP | This paper | Described in Results part 3 and Materials and methods section | |
| Recombinant DNA reagent | pcDNA3.1 NLS-(G4C2)73-GFP | This paper | Described in Results part 3 and Materials and methods section | |
| Recombinant DNA reagent | pcDNA3.1 NES-(G4C2)73-GFP | This paper | Described in Results part 3 and Materials and methods section | |
| Recombinant DNA reagent | Plasmid expressing NLS-LS |
PMID:31296649 | Prof. F. Ulrich Hartl (Max Planck Institute for Biochemistry) | |
| Recombinant DNA reagent | Plasmid expressing S-mApple |
This paper | Described in Results part 2 and Materials and methods section | |
| Recombinant DNA reagent | Plasmid expressing c-myc-NES-β17 | PMID:26634439 | Prof. F. Ulrich Hartl (Max Planck Institute for Biochemistry) | |
| Recombinant DNA reagent | Plasmid expressing c-myc-NLS-β17 | PMID:26634439 | Prof. F. Ulrich Hartl (Max Planck Institute for Biochemistry) | |
| Recombinant DNA reagent | Plasmid expressing Htt96Q |
PMID:26634439 | Prof. F. Ulrich Hartl (Max Planck Institute for Biochemistry) | |
| Antibody | Mouse monoclonal anti-nuclear pore complex proteins | Abcam | Cat. #: ab24609 RRID:AB_448181 |
IF (1:1000) |
| Antibody | Mouse monoclonal anti-NPM1 | Invitrogen | Cat. #: 32-5200 RRID:AB_2533084 |
IF (1:1000) |
| Antibody | Rabbit monoclonal anti-NF-κB p65 (D14E12) | Cell Signaling Technology | Cat. #: 8242 RRID:AB_10859369 |
IF (1:1000) |
| Antibody | Mouse monoclonal anti-p62 | Abcam | Cat. #: ab203430 RRID:AB_2728795 | IF (1:1000) |
| Antibody | Mouse monoclonal anti-c-Myc-Cy3 (9E10) | Sigma | Cat. #: C6594 RRID:AB_258958 |
IF (1:1000) |
| Antibody | Rabbit polyclonal anti-amyloid fibrils OC | Millipore | Cat. #: AB2286 RRID:AB_1977024 |
IF (1:500) |
| Antibody | Mouse biclonal anti-GFP | Roche | Cat. #: 11814460001 RRID:AB_390913 |
WB (1:1000) |
| Antibody | Mouse monoclonal anti-α-tubulin | Sigma | Cat. #: T6199 RRID:AB_477583 |
WB (1:1000) |
| Antibody | Mouse monoclonal anti-RPA40 | Santa Cruz | Cat. #: sc-374443 RRID:AB_10991310 |
IF (1:1000) |
| Antibody | Mouse monoclonal anti-IκBα (L35A5) | Cell Signaling Technology | Cat. #: 4814 RRID:AB_390781 |
WB (1:/1000) |
| Antibody | Rabbit monoclonal anti-phospho-NF-κB p65 (Ser533) (93H1) | Cell Signaling Technology | Cat. #: 3033 RRID:AB_331284 |
WB (1:1000) |
| Antibody | Rabbit polyclonal anti-KPNA2 | Abcam | Cat. #: ab70160 RRID:AB_2133673 |
IF (1:1000) |
| Antibody | Rabbit polyclonal anti-KPNA4 | Abcam | Cat. #: ab84735 RRID:AB_1860702 |
IF (1:1000) |
| Antibody | Mouse monoclonal anti-KPNB1 | Abcam | Cat. #: ab2811 RRID:AB_2133989 |
IF (1:1000) |
| Antibody | Mouse monoclonal anti-GAPDH |
Millipore | Cat. #: MAB374 RRID:AB_2107445 |
WB (1:2000) |
| Antibody | Rabbit polyclonal anti-poly-PR | Proteintech | Cat. #: 23979-1-AP RRID:AB_2879388 |
WB (1:1000) |
| Antibody | Mouse monoclonal anti-poly-GR (5A2) | Millipore | Cat. #: MABN778 RRID:AB_2728664 |
WB (1:1000) |
| Antibody | Goat Secondary anti-mouse IgG-Alexa 488 | Cell Signaling Technology | Cat. #: 4408 RRID:AB_10694704 |
IF (1:1000) |
| Antibody | Goat Secondary anti-mouse IgG-Alexa 555 | Cell Signaling Technology | Cat. #: 4409 RRID:AB_1904022 |
IF (1:1000) |
| Antibody | Goat Secondary anti-rabbit IgG-Alexa 555 | Cell Signaling Technology | Cat. #: 4413 RRID:AB_10694110 |
IF (1:1000) |
| Antibody | Secondary chicken anti-mouse IgG-Alexa 488 | Invitrogen | Cat. #: 21200 RRID:AB_2535786 |
IF (1:1000) |
| Antibody | Secondary goat anti-mouse IgG-Alexa 633 | Invitrogen | Cat. #: A21053 RRID:AB_2535720 |
IF (1:1000) |
| Antibody | Secondary goat anti-mouse IgG-peroxidase | Sigma-Aldrich | Cat. #: A4416 RRID:AB_258167 |
WB (1:10,000) |
| Antibody | Secondary goat anti-rabbit IgG-peroxidase | Sigma-Aldrich | Cat. #: A9169 RRID:AB_258434 |
WB (1:10,000) |
| Sequence-based reagent | T30 | PMID:26634439 | FISH probe | Cy5-conjugated |
| Sequence-based reagent | (C4G2)5 | This paper | FISH probe | Cy3-conjugated |
| Chemical compound, drug | UltraPure SSC buffer | Thermo Fisher Scientific | Cat. #: 15557044 | For FISH |
| Chemical compound, drug | Formamide | Sigma-Aldrich | Cat. #: 47671 | For FISH |
| Chemical compound, drug | Dextran sulphate | Sigma-Aldrich | Cat. #: D6001 | For FISH |
| Chemical compound, drug | AmyTracker 680 (AmyT) | Ebba Biotech AB | Cat. #: AmyTracker 680 | Amyloid dye |
| Chemical compound, drug | DAPI | Invitrogen | Cat. #: D1306 RRID:AB_2629482 |
Nuclear stain |
| Chemical compound, drug | Thiazolyl blue tetrazolium bromide | Sigma Aldrich | Cat. #: M2128 | For cell viability |
| Chemical compound, drug | N,N-dimethylformamide | Sigma-Aldrich | Cat. #: D4551 | For cell viability |
| Chemical compound, drug | SDS | Sigma-Aldrich | Cat. #: L3771 | For cell viability |
| Chemical compound, drug | Acetic acid | Sigma-Aldrich | Cat. #: A6283 | For cell viability |
| Chemical compound, drug | Leptomycin B | Sigma Aldrich | Cat. #: L2913 | See Materials and methods section |
| Commercial assay or kit | CellTiter-Glo 2.0 | Promega | Cat. #: G9241 | For cell viability |
| Commercial assay or kit | Lipofectamine 2000 transfection reagent | Thermo Fischer Scientific | Cat. #: 11668019 | |
| Commercial assay or kit | Click-iT Plus OPP Alexa Fluor 594 Protein Synthesis Assay Kit | Thermo Fischer Scientific | Cat. #: C10457 | |
| Peptide, recombinant protein | Human recombinant TNFα | Jena Biosciences | Cat. #: PR-430 |