Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Biological sample (Homo sapiens) | Peripheral Blood Mononuclear Cells; PBMC | Venous puncture | Freshly isolated from Homo sapiens, male and female, 18–75 years | |
Biological sample (Homo sapiens) | Bone Marrow Aspirate; Primary Leukemia | Posterior Iliac Crest | Freshly isolated from Homo sapiens | |
Biological sample (Homo sapiens) | Human Myoblast; HMB | Muscle biopsies from gastrocnemius muscle | Freshly isolated from Homo sapiens | |
Biological sample (Homo sapiens) | CD34+ Cells | HemaCare | CAT#: BM34C | Isolated freshly from bone marrow (Homo sapien, M) |
Biological sample (Homo sapiens) | CD34+ Cells | HemaCare | CAT#: BM34C | Isolated freshly from bone marrow (Homo sapien, M) |
Biological sample (Homo sapiens) | CD34+ Cells | HemaCare | CAT#: BM34C | Isolated freshly from bone marrow (Homo sapien, F) |
Biological sample (Homo sapiens) | Bone Marrow Aspirate; BM Healthy | HemaCare | CAT#: BM008F | Isolated freshly from bone marrow (Homo sapien, F) |
Biological sample (Homo sapiens) | Bone Marrow Aspirate; BM Healthy | HemaCare | CAT#: BM008F | Isolated fresh (Homo sapien, M) |
Biological sample (Homo sapiens) | Bone Marrow Aspirate; BM Healthy | HemaCare | CAT#: BM008F | Isolated fresh (Homo sapien, M) |
Cell line (Homo sapien) |
HL-60 | ATCC | CAT#: CCL-240 | |
Cell line (Homo sapien) |
MV-4–11 | ATCC | CAT#: CRL-9591 | |
Cell line (Homo sapien) |
KG-1 | ATCC | CAT#: CCL-246 | |
Chemical compound, drug | Oligomycin; Oligo | Tocris | CAT#: 4110 | |
Chemical compound, drug | FCCP | Millipore Sigma | CAT#: C2920 | |
Chemical compound, drug | Rotenone; Rot | Millipore Sigma | CAT#: R8875 | |
Chemical compound, drug | Antimycin; Ant | Millipore Sigma | CAT#: A8674 | |
Chemical compound, drug | Digitonin; Digi | Millipore Sigma | CAT#: D1410599 | |
Chemical compound, drug | Potassium Pyruvate; P; Pyr | Combi-Blocks | CAT#: QA-1116 | |
Chemical compound, drug | L-Malic Acid; Malate; M | Alpha Aesar | CAT#: A13702 | |
Chemical compound, drug | L-Glutamic Acid; Glutamate; G | RPI | CAT#: G25200 | |
Chemical compound, drug | Succinic Acid; Succinate; S; Succ | Fisher | CAT#: BP336 | |
Chemical compound, drug | Octanoyl-L-Carnitine; O | Millipore Sigma | CAT#: 50892 | |
Chemical compound, drug | Cytochrome C | Millipore Sigma | CAT#: C2506 | |
Chemical compound, drug | ATP | Ark Pharma | CAT#: AK54737 | |
Chemical compound, drug | Creatine Kinase | Millipore Sigma | CAT#: C2506 | |
Chemical compound, drug | Phosphocreatine; PCr | Millipore Sigma | CAT#: 237911 | |
Chemical compound, drug | Carboxy Atractyloside; CAT | Millipore Sigma | CAT#: C4992 | |
Chemical compound, drug | 17-AAG | Millipore Sigma | CAT#:100068 | |
Chemical compound, drug | Gamitrinib TPP hexafluorophosphate; Gamitrinib | MedChem Express | CAT#: HY-102007A | |
Chemical compound, drug | Curcumin | Millipore Sigma | CAT#: 239802 | |
Chemical compound, drug | Venetoclax | Selleckchem | CAT#: S8048 | |
Chemical compound, drug | Bongkrekic acid | Cayman Chemical | CAT#: 19079 | |
Chemical compound, drug | ACK Lysis Buffer | Lonza | CAT#: BP10-548E | |
Chemical compound, drug | Mitotracker Green-FM Dye; MTG-FM | Thermo Fisher | CAT#: M7514 | |
Chemical compound, drug | Tetramethyl rhodamine methyl ester (TMRM) | Invitrogen | CAT#: 1924079 | |
Chemical compound, drug | Pierce Lys-C Protease | Thermo Fisher | CAT#: 90307 | |
Transfected construct (Homo sapien) | shRNA to TRAP1 | Origene | CAT#: TL300868V | shRNA lentiviral particles packaged from pGFP-C-shLenti vector |
Sequence-based reagent | Primer for TRAP1 | Thermo Fisher | CAT#: 4331182 | |
Sequence-based reagent | Primer for 18 s rRNA | Thermo Fisher | CAT#: 4319413E | |
Peptide, recombinant protein | Seq Grade Trypsin | Millipore Sigma | CAT#: V5113 | |
Peptide, recombinant protein | Human Stem Cell Factor; SCF | Gibco | CAT#: PHC2115 | |
Peptide, recombinant protein | Human Thrombopoietin | Gibco | CAT#: PHC9514 | |
Peptide, recombinant protein | FLT-3 Ligand | Sigma Aldrich | CAT#: SRP3044 | |
Commercial assay or kit | RNeasy Midi kit | Qiagen | CAT#: 74124 | |
Commercial assay or kit | Superscript IV reverse transcriptase | Invitrogen | CAT#: 18090010 | |
Commercial assay or kit | TMT 10-plex | Thermo Fisher | CAT#: A34808 | |
Software, algorithm | Prism 8.4 | GraphPad | RRID:SCR_002798 | |
Software, algorithm | Proteome Discoverer 2.2 | Thermo Fisher | ||
Software, algorithm | Mito Carta 2.0 | RRID:SCR_018165 |